集胞藻6803菌株中类dnaA基因的转录分析与突变
Transcriptional analysis and mutation of a dnaA-like gene in Synechocystis sp. strain PCC 6803.
作者信息
Richter S, Hagemann M, Messer W
机构信息
Max-Planck-Institut für molekulare Genetik, D-14195 Berlin, Germany.
出版信息
J Bacteriol. 1998 Sep;180(18):4946-9. doi: 10.1128/JB.180.18.4946-4949.1998.
Abstract
Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and did not show an aberrant phenotype. We conclude that, unlike the situation in other bacteria, dnaA of Synechocystis sp. cannot have an essential function, such as initiation of DNA replication.
摘要
通过Northern分析确定，蓝藻聚球藻属PCC 6803菌株的dnaA基因转录是光依赖性的，并产生单顺反子mRNA。令人惊讶的是，dnaA失活的突变体是可存活的。在标准条件下的分批培养中，这些突变体的生长与野生型相似，并未表现出异常表型。我们得出结论，与其他细菌的情况不同，聚球藻属的dnaA不可能具有诸如启动DNA复制等基本功能。
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