胱硫醚β-合成酶基因（cysB）和丝氨酸乙酰转移酶基因（cysE）与鼠伤寒沙门氏菌对美西林的敏感性有关。
Involvement of cysB and cysE genes in the sensitivity of Salmonella typhimurium to mecillinam.
作者信息
Oppezzo O J, Antón D N
机构信息
Departamento de Radiobiología, Comisión Nacional de Energía Atómica, Buenos Aires, Argentina.
出版信息
J Bacteriol. 1995 Aug;177(15):4524-7. doi: 10.1128/jb.177.15.4524-4527.1995.
Abstract
cysB and cysE strains were obtained as spontaneous mecillinam-resistant mutants of Salmonella typhimurium. The resistance to mecillinam was caused by the cys mutations which also conferred tolerance to lethal cell shape mutations. Most, but not all, cysB and cysE mutations from other origins displayed the same behavior. Resistance was abolished by O- and N-acetylserine in cysE mutants; by thiosulfate, sulfite, and sulfide in cysB mutants; and by cysteine in both types of mutants. It is concluded that an event involved in mecillinam action requires the inducer and the activator protein of the cysteine regulon.
摘要
cysB和cysE菌株是作为鼠伤寒沙门氏菌的自发美西林耐药突变体获得的。对美西林的耐药性是由cys突变引起的，这些突变也赋予了对致死性细胞形态突变的耐受性。大多数（但不是全部）来自其他来源的cysB和cysE突变表现出相同的行为。在cysE突变体中，O-乙酰丝氨酸和N-乙酰丝氨酸可消除耐药性；在cysB突变体中，硫代硫酸盐、亚硫酸盐和硫化物可消除耐药性；在这两种类型的突变体中，半胱氨酸均可消除耐药性。得出的结论是，美西林作用所涉及的一个事件需要半胱氨酸调节子的诱导物和激活蛋白。
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