鞭毛甲基杆菌KT中氧化还原蛋白基因的克隆、测序及突变分析
Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT.
作者信息
Gak E R, Chistoserdov A Y, Lidstrom M E
机构信息
Keck Laboratories 138-78, California Institute of Technology, Pasadena 91125, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4575-8. doi: 10.1128/jb.177.15.4575-4578.1995.
Abstract
The gene cluster for methylamine utilization (mau genes) has been cloned from the obligate methylotrophic bacterium Methylobacillus flagellatum KT. Partial sequence data showed that the organization of these genes was similar to that found in Methylophilus methylotrophus W3A1-NS, including the lack of a gene for amicyanin, which had been thought to be the electron acceptor for methylamine dehydrogenase in M. flagellatum KT. However, a gene encoding azurin was discovered at the 3' end of the mau gene cluster, transcribed in the opposite orientation. A mutant with a defect in this gene showed impaired growth on methylamine, suggesting that azurin is involved in methylamine oxidation in M. flagellatum KT.
摘要
已从专性甲基营养细菌鞭毛甲基芽孢杆菌KT中克隆出甲胺利用基因簇（mau基因）。部分序列数据表明，这些基因的组织方式与嗜甲基甲基ophilus W3A1-NS中发现的相似，包括缺少编码蓝铜蛋白的基因，该基因曾被认为是鞭毛甲基芽孢杆菌KT中甲胺脱氢酶的电子受体。然而，在mau基因簇的3'端发现了一个编码天青蛋白的基因，其转录方向相反。该基因存在缺陷的突变体在甲胺上生长受损，这表明天青蛋白参与了鞭毛甲基芽孢杆菌KT中的甲胺氧化过程。
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Organization of the methylamine utilization (mau) genes in Methylophilus methylotrophus W3A1-NS.嗜甲基甲基ophilus甲基otrophus W3A1-NS中甲胺利用（mau）基因的组织。
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