Chistoserdov A Y, Tsygankov Y D, Lidstrom M E
W. M. Keck Laboratories 138-78, California Institute of Technology, Pasadena 91125.
J Bacteriol. 1991 Sep;173(18):5901-8. doi: 10.1128/jb.173.18.5901-5908.1991.
An isolated 5.2-kb fragment of Methylobacterium extorquens AM1 DNA was found to contain a gene cluster involved in methylamine utilization. Analysis of polypeptides synthesized in an Escherichia coli T7 expression system showed that five genes were present. Two of the genes encoded the large and small subunits of methylamine dehydrogenase, and a third encoded amicyanin, the presumed electron acceptor for methylamine dehydrogenase, but the function of the other two genes is not known. The order on the 5.2-kb fragment was found to be large-subunit gene, the two genes of unknown function, small-subunit gene, amicyanin gene. The gene for azurin, another possible electron acceptor in methylamine oxidation, does not appear to be present within this cluster of methylamine utilization genes.
发现甲基营养型菌株AM1（Methylobacterium extorquens AM1）DNA的一个5.2 kb分离片段含有一个参与甲胺利用的基因簇。对在大肠杆菌T7表达系统中合成的多肽进行分析表明存在五个基因。其中两个基因编码甲胺脱氢酶的大亚基和小亚基，第三个基因编码蓝铜蛋白，推测为甲胺脱氢酶的电子受体，但另外两个基因的功能尚不清楚。发现这5.2 kb片段上的顺序为大亚基基因、两个功能未知的基因、小亚基基因、蓝铜蛋白基因。在这个甲胺利用基因簇中似乎不存在作为甲胺氧化中另一个可能电子受体的天青蛋白基因。
