Vesalainen S, Lipponen P
Department of Surgery, University of Kuopio, Finland.
J Cancer Res Clin Oncol. 1995;121(7):429-33. doi: 10.1007/BF01212951.
The expression of Rb protein was analysed by immunocytochemical methods in 118 patients with T12M0 prostatic adenocarcinoma who were followed-up for more than 12 years. Rb protein was expressed in 117/118 (99%) tumours. The fraction of positive nuclei was (mean +/- SD, 92 +/- 19%) and only one tumour was completely negative for Rb protein. Abnormal expression of the Rb protein (in fewer than 90% of cells) was independent of the T category while there was a significant relationship between DNA ploidy (P = 0.0138), S-phase fraction (P = 0.0042) and expression of the Rb protein. Abnormal expression of the Rb protein had no prognostic value while T category (P = 0.0017), Gleason score (P = 0.0097), DNA ploidy (P = 0.0034), S-phase fraction (P = 0.0179) and mitotic index (P = 0.0001) were significant prognostic factors. In multivariate analysis the only independent predictor was the mitotic index [Risk ratio (RR) (95%CI) = 7.4(2.4-22.5), P = 0.004]. The results show that Rb protein immunohistochemistry has hardly any prognostic significance in prostatic adenocarcinoma whereas direct measurement of cell proliferative activity contains significant prognostic information.
采用免疫细胞化学方法分析了118例T12M0前列腺腺癌患者的Rb蛋白表达情况，这些患者的随访时间超过12年。118个肿瘤中有117个（99%）表达Rb蛋白。阳性细胞核比例为（平均值±标准差，92±19%），只有1个肿瘤Rb蛋白完全阴性。Rb蛋白的异常表达（细胞比例少于90%）与T分期无关，而DNA倍体（P = 0.0138）、S期分数（P = 0.0042）与Rb蛋白表达之间存在显著关系。Rb蛋白的异常表达无预后价值，而T分期（P = 0.0017）、Gleason评分（P = 0.0097）、DNA倍体（P = 0.0034）、S期分数（P = 0.0179）和有丝分裂指数（P = 0.0001）是显著的预后因素。多因素分析中唯一的独立预测因素是有丝分裂指数[风险比（RR）（95%可信区间）= 7.4（2.4 - 22.5），P = 0.004]。结果表明，Rb蛋白免疫组化在前列腺腺癌中几乎没有预后意义，而直接测量细胞增殖活性包含显著的预后信息。
