Botma H J, Dekker H, van Amstel P, Cairo I, van den Berg F M
Department of Pathology-H2, University of Amsterdam, The Netherlands.
J Virol Methods. 1995 May;53(1):37-45. doi: 10.1016/0166-0934(94)00174-f.
A herpes simplex virus (HSV) type 2 specific recombinant plasmid probe designated pH2S3 was constructed from non-HSV-1 crossreactive regions of the HSV-2 genome. DNA in situ hybridization on in vitro reconstructed tissue samples of sheep collagen matrix impregnated with herpes virus-infected human cells was used to demonstrate absence of crossreactivity of the pH2S3 probe with HSV-1 and varicella zoster virus under stringent posthybridization washing conditions. It was demonstrated that DNA in situ hybridization with pH2S3 allows specific detection of HSV-2 in routine formalin-fixed, paraffin-embedded patient material.
构建了一种名为pH2S3的2型单纯疱疹病毒（HSV）特异性重组质粒探针，其取材于HSV-2基因组的非HSV-1交叉反应区域。在体外重建的、用感染疱疹病毒的人细胞浸渍的羊胶原基质组织样本上进行DNA原位杂交，以证明在严格的杂交后洗涤条件下，pH2S3探针与HSV-1和水痘带状疱疹病毒不存在交叉反应。结果表明，用pH2S3进行DNA原位杂交可在常规福尔马林固定、石蜡包埋的患者材料中特异性检测HSV-2。
