Garbuglia A R, Salvi R, Di Caro A, Montella F, Di Sora F, Recchia O, Delfini C, Benedetto A
Center of Virology, S. Camillo Hospital, Rome, Italy.
J Med Virol. 1995 Jun;46(2):116-21. doi: 10.1002/jmv.1890460206.
The HIV viral burden and RNA expression in a selected group of infected, clinically non-progressor patients were investigated. Five fast-progressor patients and 10 AIDS cases were included as controls. The HIV viral load was investigated by semiquantitative polymerase chain reaction (PCR) in adherent macrophages and in genomic and extragenomic fractions of lymphocytes. HIV DNA was not found in macrophages in the non-progressor subjects, was weakly positive in 2 of 5 fast-progressors and strongly positive in most of the AIDS patients. The number of HIV proviruses found in lymphocytes of the non-progressor subjects varied from 5 to 160 copies/microgram DNA, values ten times lower than those recorded in fast-progressors and AIDS patients. The extragenomic HIV DNA (2 LTR forms) was absent or barely detectable in the lymphocytes from non-progressors and abundant in the other groups. HIV RNA was not found in the lymphocytes of all non-progressors. This may indicate that a latent state of HIV provirus exists in the lymphocytes of these subjects. To investigate this point, cultivation and stimulation with PHA (phytohemoagglutinin) and PMA (phorbol 12-myristate 13-acetate) of lymphocytes from these subjects were attempted but after 6 days HIV RNA (RT-PCR for gag region) was still absent or barely detectable in these patients. There are no other reports of the absence of HIV provirus induction in lymphocytes from infected individuals. If confirmed in a larger number of patients, such non-inducibility might serve as a predictor marker of progression of the disease.
对一组选定的已感染但临床上病情未进展的患者的HIV病毒载量和RNA表达进行了研究。纳入了5名快速进展患者和10例艾滋病病例作为对照。通过半定量聚合酶链反应（PCR）检测贴壁巨噬细胞以及淋巴细胞基因组和基因组外部分中的HIV病毒载量。在病情未进展的受试者的巨噬细胞中未发现HIV DNA，5名快速进展患者中有2名呈弱阳性，大多数艾滋病患者呈强阳性。病情未进展的受试者淋巴细胞中发现的HIV前病毒数量为5至160拷贝/微克DNA，比快速进展患者和艾滋病患者记录的值低10倍。基因组外HIV DNA（2 LTR形式）在病情未进展者的淋巴细胞中不存在或几乎检测不到，而在其他组中含量丰富。所有病情未进展者的淋巴细胞中均未发现HIV RNA。这可能表明这些受试者的淋巴细胞中存在HIV前病毒的潜伏状态。为了研究这一点，尝试对这些受试者的淋巴细胞进行培养并用PHA（植物血凝素）和PMA（佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯）刺激，但6天后这些患者中仍未发现或几乎检测不到HIV RNA（gag区域的RT - PCR）。在感染个体的淋巴细胞中未诱导出HIV前病毒的情况尚无其他报道。如果在更多患者中得到证实，这种不可诱导性可能作为疾病进展的预测标志物。
