受体结合位点缺失的口蹄疫病毒可保护牛免受口蹄疫感染。
Receptor binding site-deleted foot-and-mouth disease (FMD) virus protects cattle from FMD.
作者信息
McKenna T S, Lubroth J, Rieder E, Baxt B, Mason P W
机构信息
Plum Island Animal Disease Center, U.S. Department of Agriculture, Greenport, New York 11944, USA.
出版信息
J Virol. 1995 Sep;69(9):5787-90. doi: 10.1128/JVI.69.9.5787-5790.1995.
Abstract
Binding of foot-and-mouth disease virus (FMDV) to cells requires an arginine-glycine-aspartic acid (RGD) sequence in the capsid protein VP1. We have genetically engineered an FMDV in which these three amino acids have been deleted, producing a virus particle which is unable to bind to cells. Cattle vaccinated with these receptor binding site-deleted virions were protected from disease when challenged with a virulent virus, demonstrating that these RGD-deleted viruses could serve as the basis for foot-and-mouth disease vaccines safer than those currently in use. This strategy may prove useful in the development of vaccines for other viral diseases.
摘要
口蹄疫病毒（FMDV）与细胞的结合需要衣壳蛋白VP1中的精氨酸-甘氨酸-天冬氨酸（RGD）序列。我们通过基因工程改造了一种FMDV，其中这三个氨基酸已被删除，产生了一种无法与细胞结合的病毒颗粒。用这些缺失受体结合位点的病毒粒子接种疫苗的牛在受到强毒病毒攻击时可免受疾病侵害，这表明这些缺失RGD的病毒可作为比目前使用的疫苗更安全的口蹄疫疫苗的基础。该策略可能在其他病毒性疾病疫苗的开发中证明是有用的。
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本文引用的文献
1
CHEMICAL PHYSICAL PROPERTIES OF VIRTUALLY PURE FOOT-AND-MOUTH DISEASE VIRUS.近乎纯口蹄疫病毒的化学物理特性
Am J Vet Res. 1964 Mar;25:333-42.
2
Genetically engineered foot-and-mouth disease viruses with poly(C) tracts of two nucleotides are virulent in mice.具有两个核苷酸的聚（C）序列的基因工程口蹄疫病毒在小鼠中具有毒性。
J Virol. 1993 Sep;67(9):5139-45. doi: 10.1128/JVI.67.9.5139-5145.1993.
3
Structure of a major immunogenic site on foot-and-mouth disease virus.口蹄疫病毒主要免疫原性位点的结构
Nature. 1993 Apr 8;362(6420):566-8. doi: 10.1038/362566a0.
4
RGD sequence of foot-and-mouth disease virus is essential for infecting cells via the natural receptor but can be bypassed by an antibody-dependent enhancement pathway.口蹄疫病毒的RGD序列对于通过天然受体感染细胞至关重要，但可被抗体依赖性增强途径绕过。
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1932-6. doi: 10.1073/pnas.91.5.1932.
5
New observations on antigenic diversification of RNA viruses. Antigenic variation is not dependent on immune selection.RNA病毒抗原多样化的新观察。抗原变异不依赖于免疫选择。
J Gen Virol. 1993 Oct;74 ( Pt 10):2039-45. doi: 10.1099/0022-1317-74-10-2039.
6
Vaccines prepared from chimeras of foot-and-mouth disease virus (FMDV) induce neutralizing antibodies and protective immunity to multiple serotypes of FMDV.由口蹄疫病毒（FMDV）嵌合体制备的疫苗可诱导产生中和抗体，并对多种血清型的口蹄疫病毒产生保护性免疫。
J Virol. 1994 Nov;68(11):7092-8. doi: 10.1128/JVI.68.11.7092-7098.1994.
7
Epitopes on foot-and-mouth disease virus outer capsid protein VP1 involved in neutralization and cell attachment.口蹄疫病毒外衣壳蛋白VP1上参与中和作用及细胞附着的表位。
J Virol. 1984 Aug;51(2):298-305. doi: 10.1128/JVI.51.2.298-305.1984.
8
Foot-and-mouth disease.口蹄疫
Annu Rev Microbiol. 1968;22:201-44. doi: 10.1146/annurev.mi.22.100168.001221.
9
Sensitivity of cell cultures, cattle, mice, and guinea-pigs for detection of nineteen foot-and-mouth disease viruses.用于检测19种口蹄疫病毒的细胞培养物、牛、小鼠和豚鼠的敏感性。
Bull Off Int Epizoot. 1965 Sep-Oct;63(9):1607-25.
10
Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination.通过核苷酸序列测定对欧洲口蹄疫病毒毒株进行分型
J Virol. 1987 May;61(5):1621-9. doi: 10.1128/JVI.61.5.1621-1629.1987.
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