Tax的氨基末端是与环磷酸腺苷反应元件结合蛋白相互作用所必需的。
The amino terminus of Tax is required for interaction with the cyclic AMP response element binding protein.
作者信息
Goren I, Semmes O J, Jeang K T, Moelling K
机构信息
Max-Planck-Institut fuer Molekulare Genetik, Berlin (Dahlem), Federal Republic of Germany.
出版信息
J Virol. 1995 Sep;69(9):5806-11. doi: 10.1128/JVI.69.9.5806-5811.1995.
Abstract
Tax of human T-cell leukemia virus type 1 was analyzed for interaction with the cyclic AMP response element binding protein (CREB) in vitro with and without Tax response element DNA. Mutations in the carboxy terminus of Tax (L296G and L320G) did not affect binding to CREB and led to supershifts. In contrast, mutants with changes in the amino-terminal cysteine-rich region lost the ability to bind to CREB. The S10A mutant protein bound moderately. Thus, the amino terminus of Tax is essential for Tax-CREB interaction.
摘要
在有和没有Tax反应元件DNA的情况下，对1型人类T细胞白血病病毒的Tax进行了体外与环磷酸腺苷反应元件结合蛋白（CREB）相互作用的分析。Tax羧基末端的突变（L296G和L320G）不影响与CREB的结合，并导致超迁移。相比之下，氨基末端富含半胱氨酸区域发生变化的突变体失去了与CREB结合的能力。S10A突变蛋白结合程度适中。因此，Tax的氨基末端对于Tax与CREB的相互作用至关重要。
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