阴茎上皮内癌、原位癌、疣状癌及浸润癌中人乳头瘤病毒脱氧核糖核酸的检测

The detection of human papillomavirus deoxyribonucleic acid in intraepithelial, in situ, verrucous and invasive carcinoma of the penis.

作者信息

Cupp M R, Malek R S, Goellner J R, Smith T F, Espy M J

机构信息

Department of Urology, Mayo Clinic, Rochester, Minnesota 55905, USA.

出版信息

J Urol. 1995 Sep;154(3):1024-9.

PMID:7637047

Abstract

PURPOSE

We study the prevalence of human papillomavirus deoxyribonucleic acid (DNA) in squamous cell carcinoma and control tissue of the penis.

MATERIALS AND METHODS

The technique of polymerase chain reaction DNA amplification was used to detect specific human papillomavirus DNA sequences in archival pathological and control tissues. We analyzed 42 cases of invasive squamous cell carcinoma, 13 of carcinoma in situ, 12 of penile intraepithelial neoplasia, 3 of verrucous carcinoma and 25 of balanitis xerotica obliterans, as well as 29 routine neonatal circumcision specimens and 32 adult circumcision specimens.

RESULTS

Overall, the detection rates for human papillomavirus DNA in the study and control tissues were 55% (23 of 42 cases) for invasive squamous cell carcinoma, 92% (12 of 13) for carcinoma in situ, 92% (11 of 12) for penile intraepithelial neoplasia, 0% (0 of 3) for verrucous carcinoma, 4% (1 of 25) for balanitis xerotica obliterans, 0% (0 of 29) for neonatal circumcision and 9% (3 of 32) for adult circumcision. In all groups human papillomavirus type 16 was the most common genotype identified.

CONCLUSIONS

The prevalence of human papillomavirus DNA is significantly greater in carcinoma of the penis than in control tissue. Moreover, the prevalence is greater in noninvasive lesions (carcinoma in situ and penile intraepithelial neoplasia) than in invasive carcinoma.

摘要

目的

我们研究人乳头瘤病毒脱氧核糖核酸（DNA）在阴茎鳞状细胞癌及对照组织中的流行情况。

材料与方法

采用聚合酶链反应DNA扩增技术检测存档病理组织和对照组织中的特定人乳头瘤病毒DNA序列。我们分析了42例浸润性鳞状细胞癌、13例原位癌、12例阴茎上皮内瘤变、3例疣状癌和25例闭塞性干燥性龟头炎，以及29例常规新生儿包皮环切标本和32例成人包皮环切标本。

结果

总体而言，研究组织和对照组织中人乳头瘤病毒DNA的检出率分别为：浸润性鳞状细胞癌55%（42例中的23例），原位癌92%（13例中的12例），阴茎上皮内瘤变92%（12例中的11例），疣状癌0%（3例中的0例），闭塞性干燥性龟头炎4%（25例中的1例），新生儿包皮环切0%（29例中的0例），成人包皮环切9%（32例中的3例）。在所有组中，16型人乳头瘤病毒是最常见的基因型。