Shroyer K R, Greer R O, Fankhouser C A, McGuirt W F, Marshall R
Department of Pathology School of Medicine, University of Colorado Health Sciences, Denver 80262.
Mod Pathol. 1993 Nov;6(6):669-72.
Archival tissue biopsies of 17 cases of oral verrucous carcinoma were tested for human papillomaviruses by polymerase chain reaction followed by DNA slot-blot hybridization. Sections of the biopsies were also tested by in situ DNA hybridization with biotin-labeled probes for human papillomavirus types 6/11, 6/18, and 31/33/35. Using polymerase chain reaction, human papillomavirus DNA was detected in seven of 17 cases (41%). Slot-blot hybridization of the amplification products identified human papillomavirus type 6 in two cases, type 11 in four cases, and both types 6 and 11 in one case of oral verrucous carcinoma. In situ hybridization confirmed the presence of human papillomavirus type 6/11 DNA in tumor cell nuclei for each of the cases which tested positive by polymerase chain reaction and was negative in the remaining 10 cases. We conclude that human papillomavirus types 6 and 11 are frequently present in cases of oral verrucous carcinoma.
通过聚合酶链反应继以DNA斑点杂交，对17例口腔疣状癌的存档组织活检标本进行人乳头瘤病毒检测。活检标本切片也采用生物素标记的人乳头瘤病毒6/11型、6/18型和31/33/35型探针进行原位DNA杂交检测。采用聚合酶链反应，在17例中的7例（41%）检测到了人乳头瘤病毒DNA。扩增产物的斑点杂交在2例中鉴定出6型人乳头瘤病毒，4例中鉴定出11型，1例口腔疣状癌中同时鉴定出6型和11型。原位杂交证实，在通过聚合酶链反应检测为阳性的每例肿瘤细胞核中均存在6/11型人乳头瘤病毒DNA，其余10例为阴性。我们得出结论，6型和11型人乳头瘤病毒在口腔疣状癌病例中经常存在。
