Cinatl J, Cinatl J, Weber B, Rabenau H, Gümbel H O, Kornhuber B, Doerr H W
Centre of Hygiene, J.W. Goethe University, Frankfurt a. M. Germany.
Res Virol. 1995 Mar-Apr;146(2):125-9. doi: 10.1016/0923-2516(96)81081-8.
The human T-cell line JJHan, commonly used as a substrate for human herpesvirus type 6 (HHV-6) replication, was established in a protein-free 1:1 mixture of Ham's F12 and Iscove's modified Dulbecco's medium. After 250 passages (4 years) in protein-free medium, the cells, designated JJHan-PF, were cocultured with JJHan cells chronically infected with strain AJ of HHV-6 conventionally grown in serum-supplemented medium. The production of viral antigen was monitored by indirect immunofluorescent antibody staining. The infectivity of the virus in JJHan-PF cells was similar to that in JJHan cultures as shown by HHV-6 antigen detection using a convalescent phase serum. The results show that JJHan-PF cells provide a sensitive substrate for HHV-6 replication without the interference of serum or other proteins usually added to the culture medium.
人T细胞系JJHan通常用作人疱疹病毒6型（HHV-6）复制的底物，它是在Ham's F12和Iscove改良的杜尔贝科培养基按1:1比例混合的无蛋白培养基中建立的。在无蛋白培养基中传代250次（4年）后，将这些细胞（命名为JJHan-PF）与慢性感染HHV-6 AJ株的JJHan细胞进行共培养，HHV-6 AJ株在添加血清的培养基中常规培养。通过间接免疫荧光抗体染色监测病毒抗原的产生。如使用恢复期血清进行HHV-6抗原检测所示，病毒在JJHan-PF细胞中的感染性与在JJHan培养物中的感染性相似。结果表明，JJHan-PF细胞为HHV-6复制提供了一个敏感的底物，而不受通常添加到培养基中的血清或其他蛋白质的干扰。
