Pallon J, Malmqvist K
Lund Institute of Technology, Department of Nuclear Physics, Sweden.
Scanning Microsc Suppl. 1994;8:317-24.
The continuing development of the Nuclear Microprobe (NMP) has opened up new fields of applications in biology and medicine. Quantitative multielemental analysis in small sections of samples can be performed routinely. The use of techniques such as scanning transmission ion microscopy makes imaging as well as mass normalization possible at submicron resolution. Recent medical applications include studies on thin cryosections prepared from autopsies and biopsies, as well as single cells grown directly on the backing foil used in the NMP analyses. The purpose of the single cell analysis is often pharmacological, e.g., testing of new drugs, their uptake and distribution. New applications, for instance, in food chemistry, ecology and evolutionary genetics, are also taking advantage of the high analytical sensitivity of the NMP in combination with its imaging capability.
核微探针（NMP）的持续发展为生物学和医学开辟了新的应用领域。现在可以常规地对小样本切片进行定量多元素分析。诸如扫描透射离子显微镜等技术的应用，使得在亚微米分辨率下进行成像以及质量归一化成为可能。最近的医学应用包括对尸检和活检所得薄冰冻切片的研究，以及对直接生长在NMP分析所用支撑箔上的单细胞的研究。单细胞分析的目的通常是药理学方面的，例如测试新药、它们的摄取和分布。例如，在食品化学、生态学和进化遗传学等新应用中，也在利用NMP的高分析灵敏度及其成像能力。
