Bladbjerg E M, Overgaard K, Gram J, Jespersen J
Institute for Thrombosis Research, South Jutland University Centre, Esbjerg, Denmark.
Scand J Clin Lab Invest. 1995 May;55(3):267-71. doi: 10.3109/00365519509089623.
In the Northwick Park Heart Study, the coagulant activity of factor VII (FVII:C) has been identified as a risk marker of ischaemic heart disease. In the fasting state, the protein concentration of FVII (FVII:Ag) might be an even better risk marker, because of the low coefficient of variation of the antigen assay. Today, most analyses are performed in plasma samples, as it is unknown whether FVII, to some extent, is consumed during coagulation. In the present study, we have investigated, whether FVII:Ag can be measured equally well in plasma and serum. FVII:Ag was measured in 88 plasma and serum samples. Results were compared by means of linear regression, where y = 0.984 x +0.770, r = 0.96. No systematic variation existed between FVII:Ag in plasma and serum. The mean difference in FVII:Ag between plasma and serum was -1.17 (SD 11.92) arbitrary units, compared with a mean difference of 0.18 (SD 8.31) arbitrary units between duplicate measurements of the same plasma dilution. Our findings indicate that there is a good agreement between FVII:Ag in plasma and serum.
在诺斯威克公园心脏研究中，凝血因子VII的凝血活性（FVII:C）已被确定为缺血性心脏病的一个风险标志物。在空腹状态下，由于抗原检测的变异系数较低，凝血因子VII的蛋白浓度（FVII:Ag）可能是一个更好的风险标志物。如今，大多数分析是在血浆样本中进行的，因为尚不清楚凝血因子VII在某种程度上是否会在凝血过程中被消耗。在本研究中，我们调查了FVII:Ag在血浆和血清中的测量结果是否同样良好。我们对88份血浆和血清样本中的FVII:Ag进行了测量。通过线性回归比较结果，其中y = 0.984x + 0.770，r = 0.96。血浆和血清中的FVII:Ag之间不存在系统性差异。血浆和血清中FVII:Ag的平均差异为 -1.17（标准差11.92）个任意单位，而同一血浆稀释度的重复测量之间的平均差异为0.18（标准差8.31）个任意单位。我们的研究结果表明，血浆和血清中的FVII:Ag之间具有良好的一致性。
