Mendonça M, Da Luz M M, Freire-Maia L, Cunha-Melo J R
Departamento de Ginecologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Toxicon. 1995 Mar;33(3):355-61. doi: 10.1016/0041-0101(94)00162-2.
Scorpion toxin T1 from Tityus serrulatus was tested for its effects on the isolated rat uterus preparation. T1 (5 micrograms/ml) caused a contraction of the uterus, which was potentiated by neostigmine (1.64 x 10(-6) M) and abolished by atropine (1.4 x 10(-7) M). After addition of neostigmine to the bath, we noted a higher amplitude of the toxin-induced contractions, and the appearance of repetitive rhythmic contractions. The scorpion toxin-induced contraction was not prevented by previous addition to the bath of hexamethonium or bradykinin, 5-HT and angiotensin II antagonists. The uterine contraction was prevented by previous addition to the bath of either tetrodotoxin (5 x 10(-8) M) or lidocaine (4.2 x 10(-5) M). These data seem to indicate that scorpion toxin-induced rat uterus contractions are due to actions on post-ganglionic autonomic nerve endings, with acetylcholine release and stimulation of muscarinic receptors.
对来自锯齿钝尾蝎的蝎毒素T1对离体大鼠子宫标本的作用进行了测试。T1（5微克/毫升）引起子宫收缩，新斯的明（1.64×10⁻⁶ M）可增强该收缩，而阿托品（1.4×10⁻⁷ M）可消除该收缩。向浴槽中加入新斯的明后，我们注意到毒素诱导的收缩幅度更高，并且出现了重复性节律性收缩。预先向浴槽中加入六甲铵或缓激肽、5-羟色胺和血管紧张素II拮抗剂并不能阻止蝎毒素诱导的收缩。预先向浴槽中加入河豚毒素（5×10⁻⁸ M）或利多卡因（4.2×10⁻⁵ M）可阻止子宫收缩。这些数据似乎表明，蝎毒素诱导的大鼠子宫收缩是由于对节后自主神经末梢的作用，伴有乙酰胆碱释放和毒蕈碱受体的刺激。
