一种用于测量黄热病疫苗传染性的快速检测方法。

A rapid test for measuring the infectivity of Yellow Fever vaccine.

作者信息

Sood D K, Aggarwal R K, Kumar S, Sokhey J

机构信息

Central Drugs Laboratory, Central Research Institute, Kasauli- India.

出版信息

Vaccine. 1995 Apr;13(5):427-8. doi: 10.1016/0264-410x(94)00035-l.

DOI:10.1016/0264-410x(94)00035-l

PMID:7639009

Abstract

An in-house reference preparation of 17D-204 Yellow Fever vaccine was tested 60 times using LD50 mouse assay and CCID50 method. The mean virus titre, standard deviation and coefficient of variation obtained were comparable. Student's t-test indicated that there was a statistically insignificant difference between the two methods (p > 0.05) used for potency test with a correlation coefficient of 0.666 (p < 0.05). CCID50 assay for measuring the infectivity of Yellow Fever vaccine is rapid, sensitive and reproducible.

摘要

使用半数致死量（LD50）小鼠试验和半数细胞感染量（CCID50）方法对一种内部制备的17D - 204黄热病疫苗参考制剂进行了60次测试。获得的平均病毒滴度、标准差和变异系数具有可比性。学生t检验表明，用于效力测试的两种方法之间在统计学上无显著差异（p > 0.05），相关系数为0.666（p < 0.05）。用于测量黄热病疫苗感染性的CCID50试验快速、灵敏且可重复。