Hayashi Y, Hamano H, Haneji N, Ishimaru N, Yanagi K
Department of Pathology, Tokushima University School of Dentistry, Japan.
Arthritis Rheum. 1995 Aug;38(8):1077-84. doi: 10.1002/art.1780380809.
To analyze the repertoire of T cell receptor (TCR) V beta gene transcribed and expressed within the autoimmune lesions of the salivary gland in the MRL/lpr mouse model of Sjögren's syndrome.
Monoclonal antibodies (MAb) were used to determine the prevalence of selected V gene elements on T cell infiltrates from salivary glands of MRL/lpr mice. To analyze TCR V beta gene usage, we used reverse-transcriptase polymerase chain reaction (RT-PCR) and single-strand conformational polymorphism (SSCP) analyses.
A predominance of V beta 8+ T cells was detected within the inflammatory lesions during development of autoimmune disease (confirmed by flow cytometry). RT-PCR analysis revealed that in autoimmune sialadenitis, the predominant expression of the V beta 8 gene segment began in the early stages of disease (2-month-old mice) and increased over time. Extensive age-related diversity of TCR V beta gene usage was also observed. SSCP analysis demonstrated a distinct and common binding pattern of the V beta 8 gene PCR product from the cell infiltrates during the course of the disease.
Our data suggest that in the MRL/lpr mouse model of Sjögren's syndrome, there is restricted usage of TCR V beta elements according to the stage of the disease, and that V beta 8 are probably used preferentially in the recognition of a single unknown self antigen in the salivary gland.
分析干燥综合征MRL/lpr小鼠模型唾液腺自身免疫性病变中转录并表达的T细胞受体（TCR）Vβ基因库。
使用单克隆抗体（MAb）来确定MRL/lpr小鼠唾液腺T细胞浸润中选定V基因元件的流行情况。为了分析TCR Vβ基因的使用情况，我们采用了逆转录聚合酶链反应（RT-PCR）和单链构象多态性（SSCP）分析。
在自身免疫性疾病发展过程中的炎性病变内检测到Vβ8+ T细胞占优势（通过流式细胞术证实）。RT-PCR分析显示，在自身免疫性涎腺炎中，Vβ8基因片段的主要表达始于疾病早期（2月龄小鼠），并随时间增加。还观察到TCR Vβ基因使用存在广泛的年龄相关多样性。SSCP分析表明，在疾病过程中，来自细胞浸润的Vβ8基因PCR产物具有独特且常见的结合模式。
我们的数据表明，在干燥综合征的MRL/lpr小鼠模型中，根据疾病阶段，TCR Vβ元件的使用受到限制，并且Vβ8可能在识别唾液腺中单一未知自身抗原时被优先使用。
