类风湿性关节软骨的组织病理学和组织酶学研究。

Histopathological and histoenzimological investigations of the rheumatoid articular cartilage.

作者信息

Ciobanu A, Ciobanu I R, Hălălău F, Laky D, Dinulescu I, Stănculescu D, Stoicescu M, Stroescu I

机构信息

Victor Babeş Institute.

出版信息

Rom J Morphol Embryol. 1994 Jan-Jun;40(1-2):23-7.

PMID:7640371

Abstract

Twenty seven biopsies of articular cartilage taken intraoperatively from patients with Rheumatoid arthritis (RA) and from control patients with traumas were examined using histopathological techniques (HE, VG, PAS-Alcian, Gömöri, Safranine 0) and histoenzymological techniques (Acid phosphatase-lysomal marker, Chondroitinsulphatase, Peroxidase). Histopathologically, the rheumatoid articular cartilage appears with superficial and deep cartilaginous fissures, frequent perichondrocytic gaps associated with modification of the tinctorial activity. At the pannus synovia-cartilage junction we found invasive and destructive synovial inflammatory infiltrates penetrating and eroding the cartilage. Histoenzymologically, the rheumatoid chondrocytes have a high lysosomal potential (phosphatasic, chondroitinsulphatasic) and highly oxidative potential (peroxidasic) specific for lesion modifications.

摘要

采用组织病理学技术（苏木精-伊红染色、维多利亚蓝染色、过碘酸-阿尔辛蓝染色、Gömöri染色、番红O染色）和组织酶学技术（酸性磷酸酶-溶酶体标记物、硫酸软骨素酶、过氧化物酶），对27例类风湿关节炎（RA）患者术中获取的关节软骨活检标本以及创伤对照患者的标本进行了检查。组织病理学上，类风湿性关节软骨表现为浅表和深部软骨裂隙，常见软骨周细胞间隙并伴有染色活性改变。在血管翳-滑膜-软骨交界处，我们发现侵袭性和破坏性滑膜炎症浸润穿透并侵蚀软骨。组织酶学上，类风湿性软骨细胞具有高溶酶体活性（磷酸酶、硫酸软骨素酶活性）和高氧化活性（过氧化物酶活性），这是病变改变的特异性表现。