Stam R, Kroese A B, Croiset G, Wiegant V M, Akkermans L M
Rudolf Magnus Institute, Department of Medical Pharmacology, Utrecht University, The Netherlands.
J Pharmacol Toxicol Methods. 1995 Jun;33(3):129-36. doi: 10.1016/1056-8719(94)00067-e.
Myoelectric activity of the small intestine was recorded digitally in fasted, freely moving rats fitted with multiple pairs of electrodes in the antimesenterial smooth muscle. The electrodes were implanted under strict aseptic conditions to safeguard the animal's health and thereby benefit experimental results. As in many other mammalian species, the fasted electrical and mechanical activity of the small intestine in the rat consists of alternating periods of activity and quiescence jointly called the Migrating Motility Complex (MMC). Existing methods for the analysis of small bowel myoelectric activity either integrate spike activity over longer periods of time or describe characteristics of single bursts. We have developed a computer program for fast, automated analysis that distinguishes the three characteristic phases of the MMC. The computer program recognizes periods of varying spike burst frequency and then produces a graphical and numerical output of the length and mean burst frequency of the different phases of each MMC that correlates well with the results of visual evaluation. The techniques used are discussed in relation to existing recording and analysis methods, and specific decisions in the program are motivated. The system will be a useful tool in assessing the effects of drugs, peptides, or stress on small bowel motility.
在禁食的、自由活动的大鼠空肠系膜平滑肌上安装多对电极，以数字方式记录小肠的肌电活动。电极在严格的无菌条件下植入，以保障动物健康，从而有利于实验结果。与许多其他哺乳动物一样，大鼠小肠禁食时的电活动和机械活动由活动期和静止期交替组成，统称为移行性运动复合波（MMC）。现有的小肠肌电活动分析方法要么在较长时间内对锋电位活动进行积分，要么描述单个爆发的特征。我们开发了一个用于快速自动分析的计算机程序，该程序可区分MMC的三个特征阶段。该计算机程序识别不同锋电位爆发频率的时期，然后生成每个MMC不同阶段的长度和平均爆发频率的图形和数值输出，这与视觉评估结果高度相关。结合现有的记录和分析方法讨论了所使用的技术，并说明了程序中的具体决策依据。该系统将成为评估药物、肽或应激对小肠运动影响的有用工具。
