Pöch G, Pancheva S N
Department of Pharmacology and Toxicology, University of Graz, Austria.
J Pharmacol Toxicol Methods. 1995 Jun;33(3):137-45. doi: 10.1016/1056-8719(94)00068-f.
Comparing dose-response curves (DRCs) of a compound A in the absence and presence of a fixed dose of an antagonist B is standard in pharmacology and toxicology. When B qualitatively resembles A in its action, it is often useful to construct theoretical DRCs of additive and independent combinations. Theoretical curves are calculated from experimental values by the program ALLFIT, which uses the four parameter logistic equation. DRCs of theoretical, additive DRCs are obtained by using the respective values for slope and ED50, which were taken from tables presented here compiled on the basis of the slope of the DRC of A alone (0.6-14) and of the effect of B alone (1-75%). These tables are unnecessary for the construction of theoretical curves if A acts by an independent mechanism, giving values for slope and ED50 identical to those of the DRC of A alone. Experimental DRCs of antiviral and other effects (the latter taken from data in the literature) are compared with theoretical curves by an F-test analysis provided by ALLFIT. The method can be used successfully for the construction of theoretical curves for additive and independent DRCs and comparison with experimental curves. This comparison may help clarify the mode of interaction of A with B.
在药理学和毒理学中，比较化合物A在不存在和存在固定剂量拮抗剂B时的剂量反应曲线（DRC）是标准操作。当B在作用上与A定性相似时，构建相加和独立组合的理论DRC通常很有用。理论曲线由ALLFIT程序根据实验值计算得出，该程序使用四参数逻辑方程。理论相加DRC的曲线通过使用斜率和ED50的相应值获得，这些值取自此处呈现的表格，这些表格是根据单独的A的DRC斜率（0.6 - 14）和单独的B的效应（1 - 75%）汇编而成。如果A通过独立机制起作用，给出的斜率和ED50值与单独的A的DRC相同，那么构建理论曲线时这些表格就不必要了。通过ALLFIT提供的F检验分析，将抗病毒和其他效应的实验DRC（后者取自文献数据）与理论曲线进行比较。该方法可成功用于构建相加和独立DRC的理论曲线，并与实验曲线进行比较。这种比较可能有助于阐明A与B的相互作用模式。
