Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain.
Fred Hutchinson Cancer Research Center (retired), Seattle, WA 98109, USA.
Toxins (Basel). 2020 May 9;12(5):308. doi: 10.3390/toxins12050308.
Ciguatoxins (CTXs) are a group of neurotoxins responsible for the syndrome ciguatera fish poisoning (CFP) as a result of the consumption of contaminated fish. The presence of these toxins has been detected around the Pacific, Caribbean and Indian coasts. Recent reports indicate the emergence of CFP in other geographic areas, in particular in European coasts, of the Canary Islands (Spain) and Madeira (Portugal). A neuroblastoma cell line of murine origin (N2a) has been applied to assay different groups of neurotoxins, acting on voltage-gated sodium channel (VGSC) of excitable cells, N2a-MTT. The great potential of N2a-MTT as a sensitive tool for the CTXs screening is clearly recognized, notably because it allows the detection of these toxins at levels below recommended as security levels. However, the complexity of the matrix is a critical point on the application of N2a-MTT, which needs to be evaluated. The aim of this work is to provide recommendations for an implemented N2a-MTT method for CTXs determination in fish that avoids matrix effects, particularly those related to high lipid content.
雪卡毒素（CTXs）是一组神经毒素，由于食用受污染的鱼类而导致雪卡鱼中毒（CFP）综合征。这些毒素在太平洋、加勒比海和印度洋沿岸都有发现。最近的报告表明，CFP 在其他地理区域出现，特别是在西班牙的加那利群岛和葡萄牙的马德拉岛的欧洲沿海地区。一种源自鼠源的神经母细胞瘤细胞系（N2a）已应用于检测不同组的神经毒素，这些毒素作用于可兴奋细胞的电压门控钠离子通道（VGSC），即 N2a-MTT。N2a-MTT 作为 CTXs 筛选的敏感工具具有巨大的潜力，这一点已得到明确认可，尤其是因为它允许在低于推荐安全水平的水平检测到这些毒素。然而，基质的复杂性是 N2a-MTT 应用的一个关键点，需要对其进行评估。本工作旨在为避免基质效应，特别是与高脂质含量相关的基质效应，提供在鱼类中测定 CTXs 的实施 N2a-MTT 方法的建议。
