Rubin J S, Bottaro D P, Chedid M, Miki T, Ron D, Cheon G, Taylor W G, Fortney E, Sakata H, Finch P W
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.
Cell Biol Int. 1995 May;19(5):399-411. doi: 10.1006/cbir.1995.1085.
Keratinocyte growth factor (KGF) is a member of the heparin-binding fibroblast growth factor family (FGF-7) with a distinctive pattern of target-cell specificity. Studies performed in cell culture suggested that KGF was mitogenically active only on epithelial cells, albeit from a variety of tissues. In contrast, KGF was produced solely by cells of mesenchymal origin, leading to the hypothesis that it might function as a paracrine mediator of mesenchymal-epithelial communication. Biochemical analysis and molecular cloning established that the KGF receptor (KGFR) was a tyrosine kinase isoform encoded by the fgfr-2 gene. Many detailed investigations of KGF and KGFR expression in whole tissue and cell lines largely substantiated the pattern initially perceived in vitro of mesenchymal and epithelial distribution, respectively. Moreover, functional assays in organ culture and in vivo and studies of KGF regulation by sex steroid hormones reinforced the idea that KGF acts predominantly on epithelial cells to elicit a variety of responses including proliferation, migration and morphogenesis.
角质形成细胞生长因子（KGF）是肝素结合成纤维细胞生长因子家族（FGF - 7）的成员，具有独特的靶细胞特异性模式。在细胞培养中进行的研究表明，KGF仅对上皮细胞具有促有丝分裂活性，尽管这些上皮细胞来自多种组织。相比之下，KGF仅由间充质来源的细胞产生，这导致了一种假设，即它可能作为间充质 - 上皮细胞通讯的旁分泌介质发挥作用。生化分析和分子克隆证实，KGF受体（KGFR）是由fgfr - 2基因编码的酪氨酸激酶同工型。对全组织和细胞系中KGF和KGFR表达的许多详细研究在很大程度上证实了最初在体外观察到的间充质和上皮分布模式。此外，器官培养和体内的功能测定以及性甾体激素对KGF调节的研究强化了这样一种观点，即KGF主要作用于上皮细胞，引发包括增殖、迁移和形态发生在内的多种反应。
