Naka A, Yamamoto K, Albert M J, Honda T
Research Institute for Microbial Diseases, Osaka University, Japan.
FEMS Immunol Med Microbiol. 1995 Apr;11(2):87-90. doi: 10.1111/j.1574-695X.1995.tb00093.x.
Haemaglutinin/protease (HA/P) is one of the virulence factors of Vibrio cholerae O1 and pathogenic strains of V. cholerae non-O1. In this study, we examined protease activity of a new serogroup of Vibrio cholerae recently designated as O139 synonym Bengal. The protease activity was produced by all eight isolates of V. cholerae O139 from Bangladeshi patients. Purification and partial characterization of the protease from V. cholerae O139 demonstrated the purified protease (O139-P) was indistinguishable from that previously reported for HA/P of V. cholerae non-O1 (NAG-HA/P) and V. cholerae O1 (Vc-HA/P). These results prove that V. cholerae O139 produces a protease belonging to HA/P, and suggest that the protease is another virulence factor found in newly emerged V. cholerae O139, as in V. cholerae O1.
血凝素/蛋白酶（HA/P）是霍乱弧菌O1和非O1霍乱弧菌致病菌株的毒力因子之一。在本研究中，我们检测了最近被命名为O139（同义词孟加拉型）的新型霍乱弧菌血清群的蛋白酶活性。来自孟加拉国患者的所有8株霍乱弧菌O139均产生了蛋白酶活性。对霍乱弧菌O139蛋白酶的纯化及部分特性分析表明，纯化后的蛋白酶（O139-P）与先前报道的非O1霍乱弧菌（NAG-HA/P）和霍乱弧菌O1（Vc-HA/P）的HA/P无法区分。这些结果证明霍乱弧菌O139产生了一种属于HA/P的蛋白酶，表明该蛋白酶是新出现的霍乱弧菌O139中发现的另一种毒力因子，如同在霍乱弧菌O1中一样。
