Samowitz W S, Thliveris A, Spirio L N, White R
Department of Pathology, University of Utah, Salt Lake City 84132, USA.
Cancer Res. 1995 Sep 1;55(17):3732-4.
Reverse transcription-PCR combined with either (a) restriction enzyme digestion and repeat PCR or (b) ligase chain reaction has identified two new alternatively spliced transcripts of the adenomatous polyposis coli (APC) gene. In one of these transcripts exons 1-4 and the first 16 bases of exon 5 are deleted; in the other exons 2-4 and the first 16 bases of 5 are deleted. Both transcripts use an intraexonic splice acceptor in exon 5. These transcripts delete exons mutated in attenuated APC (3 and 4) and could account for the reduction in severity of this variant.
逆转录聚合酶链反应（Reverse transcription-PCR）与以下两种方法之一相结合：（a）限制性内切酶消化和重复PCR，或（b）连接酶链式反应，已鉴定出腺瘤性息肉病大肠杆菌（APC）基因的两种新的可变剪接转录本。在其中一种转录本中，外显子1-4和外显子5的前16个碱基被删除；在另一种转录本中，外显子2-4和外显子5的前16个碱基被删除。两种转录本均使用外显子5中的外显子内剪接受体。这些转录本删除了在弱化型APC中发生突变的外显子（3和4），这可能解释了该变体严重程度的降低。
