人肝脏中心房钠尿肽受体-A的mRNA表达：采用逆转录聚合酶链反应检测

Expression of mRNA for atrial natriuretic peptide receptor-A in human liver: detection using RT-PCR.

作者信息

Pardhasaradhi K, Kutty R K, Bertolotti R, Krishna G

机构信息

Section on Drug-Tissue Interaction, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Drug Metab Rev. 1995;27(1-2):231-9. doi: 10.3109/03602539509029824.

DOI:10.3109/03602539509029824

PMID:7641577

Abstract

cDNA preparations obtained from human liver poly A+ RNA by reverse transcription were subjected to polymerase chain reaction. Primers used in the reaction were designed from the reported cDNA sequence for human placental atrial natriuretic peptide receptor-A (ANPRA). Sequence analysis of the amplified product (approximately 700 bp) showed 100% identity to the 2476-3189 bp region of the reported cDNA sequence of human placental ANPRA. Northern blot analysis of human liver poly A+ RNA fractions showed a hybridization signal at approximately 4.4 kb, identical to the signal obtained from the poly A+ RNA fractions of human placenta. These results indicate the possible mRNA expression for atrial natriuretic peptide receptor-A in human liver.

摘要

通过逆转录从人肝脏多聚腺苷酸加尾RNA（poly A+ RNA）获得的互补DNA（cDNA）制剂进行了聚合酶链反应。反应中使用的引物是根据报道的人胎盘心钠素受体-A（ANPRA）的cDNA序列设计的。扩增产物（约700碱基对）的序列分析显示，与报道的人胎盘ANPRA的cDNA序列的2476 - 3189碱基对区域有100%的同一性。人肝脏多聚腺苷酸加尾RNA组分的Northern印迹分析显示在约4.4千碱基处有杂交信号，与从人胎盘的多聚腺苷酸加尾RNA组分获得的信号相同。这些结果表明人肝脏中可能存在心钠素受体-A的信使核糖核酸（mRNA）表达。