核因子κB p50：DNA复合物的结构：分析Rel家族的起点。

The structure of the NF-kappa B p50:DNA-complex: a starting point for analyzing the Rel family.

作者信息

Müller C W, Harrison S C

机构信息

Howard Hughes Medical Institute, Cambridge, MA 02138, USA.

出版信息

FEBS Lett. 1995 Aug 1;369(1):113-7. doi: 10.1016/0014-5793(95)00541-g.

DOI:10.1016/0014-5793(95)00541-g

PMID:7641872

Abstract

The Rel family comprises a group of structurally related, eukaryotic transcription factors. The similarity extends over about 300 amino acid residues, the Rel homology region, which is responsible for DNA binding and dimerization. Two independently determined structures of homodimeric NF-kappa B p50 bound to DNA show the Rel homology regions and the DNA target sites. The protein consists of two beta-barrel domains connected by a short linker. Five loops per monomer contact the DNA. Different half-site spacings in the two structures lead to different relative orientations of N- and C-terminal domains.

摘要

Rel家族由一组结构相关的真核转录因子组成。这种相似性延伸至约300个氨基酸残基，即Rel同源区域，它负责DNA结合和二聚化。与DNA结合的同型二聚体NF-κB p50的两个独立测定结构显示了Rel同源区域和DNA靶位点。该蛋白质由两个通过短连接子相连的β-桶状结构域组成。每个单体有五个环与DNA接触。两种结构中不同的半位点间距导致N端和C端结构域的相对取向不同。