编码非洲爪蟾热休克因子1（XHSF1）的cDNA：核苷酸序列、推导的氨基酸序列以及所编码蛋白质的特性。
The cDNA encoding Xenopus laevis heat-shock factor 1 (XHSF1): nucleotide and deduced amino-acid sequences, and properties of the encoded protein.
作者信息
Stump D G, Landsberger N, Wolffe A P
机构信息
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892-2710, USA.
出版信息
Gene. 1995 Jul 28;160(2):207-11. doi: 10.1016/0378-1119(95)00176-7.
We have isolated a cDNA encoding Xenopus laevis (Xl) heat-shock factor 1 (XHSF1). XHSF1, translated from the mRNA synthesized in vitro, will bind specifically to the Xl hsp70 promoter (hsp70). Microinjection of XHSF1 mRNA into Xl oocytes leads to synthesis of XHSF1 which accumulates in the nucleus and selectively activates Xl phsp70p activity at 18 degrees C.
我们分离出了一种编码非洲爪蟾（Xenopus laevis，Xl）热休克因子1（XHSF1）的cDNA。从体外合成的mRNA翻译而来的XHSF1，将特异性结合到Xl hsp70启动子（hsp70）上。将XHSF1 mRNA显微注射到Xl卵母细胞中会导致XHSF1的合成，其在细胞核中积累，并在18摄氏度时选择性激活Xl phsp70p活性。
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