非洲爪蟾xSox7 cDNA的克隆与特性分析

Cloning and characterization of Xenopus laevis xSox7 cDNA.

作者信息

Shiozawa M, Hiraoka Y, Komatsu N, Ogawa M, Sakai Y, Aiso S

机构信息

Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1996 Nov 11;1309(1-2):73-6. doi: 10.1016/s0167-4781(96)00145-5.

DOI:10.1016/s0167-4781(96)00145-5

PMID:8950180

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shif assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

摘要

一类与SRY相关的基因被命名为SOX。我们从非洲爪蟾卵巢中分离并测序了一个编码新型Sox蛋白的cDNA。该cDNA包含一个编码362个氨基酸的开放阅读框（ORF），其中含有一个HMG框，与小鼠Sox7的HMG框具有90%的高度同源性；在本研究中该cDNA被命名为xSox7。Northern印迹分析显示，xSox7 mRNA的长度为2.0 kb。通过逆转录/聚合酶链反应检测了成年青蛙的各种组织中的xSox7 mRNA，结果表明xSox7在多种组织中均有表达。此外，电泳迁移率变动分析表明，重组xSox7能够与AACAAT序列结合。