粟酒裂殖酵母中一个预测编码钾离子转运蛋白的基因SpTRK的分离与鉴定

Isolation and characterization of SpTRK, a gene from Schizosaccharomyces pombe predicted to encode a K+ transporter protein.

作者信息

Soldatenkov V A, Velasco J A, Avila M A, Dritschilo A, Notario V

机构信息

Department of Radiation Medicine, Georgetown University Medical Center, Washington, DC 20007, USA.

出版信息

Gene. 1995 Aug 8;161(1):97-101. doi: 10.1016/0378-1119(95)00274-a.

DOI:10.1016/0378-1119(95)00274-a

PMID:7642145

Abstract

A novel gene, SpTRK, has been isolated from DNA of the fission yeast Schizosaccharomyces pombe (Sp) by hybridization to an oligodeoxyribonucleotide (oligo) probe designed from a sequence fully conserved between the potassium transporter genes TRK1 and TRK2 of Saccharomyces cerevisiae (Sc). SpTRK is a single-copy gene located on Sp chromosome I. Nucleotide sequence analysis of the cloned gene identified an open reading frame (ORF) with coding capacity for a protein of 833 amino acids (aa). The predicted SpTRK aa sequence showed a high level of conservation relative to the potassium transporters of Sc and Saccharomyces uvarum (Su), particularly within their transmembrane (TM) domains and in aa required for their ion transport functions. A single SpTRK transcript of about 2.7 kb is expressed at high levels in exponentially growing Sp cells, but it is downregulated in cells from stationary cultures.

摘要

通过与根据酿酒酵母（Sc）的钾转运蛋白基因TRK1和TRK2之间完全保守的序列设计的寡脱氧核糖核苷酸（oligo）探针杂交，从裂殖酵母粟酒裂殖酵母（Sp）的DNA中分离出一个新基因SpTRK。SpTRK是位于Sp染色体I上的单拷贝基因。对克隆基因的核苷酸序列分析确定了一个开放阅读框（ORF），其编码能力为一个833个氨基酸（aa）的蛋白质。预测的SpTRK氨基酸序列与Sc和葡萄汁酵母（Su）的钾转运蛋白相比具有高度保守性，特别是在它们的跨膜（TM）结构域和离子转运功能所需的氨基酸中。一个约2.7 kb的单一SpTRK转录本在指数生长的Sp细胞中高水平表达，但在静止培养的细胞中表达下调。