Couzin N, Trézéguet V, Le Saux A, Lauquin G J
IBGC/CNRS, Bordeaux, France.
Gene. 1996 May 24;171(1):113-7. doi: 10.1016/0378-1119(96)00095-9.
We describe the isolation and sequencing of both cDNA and genomic clones encoding the mitochondrial ADP/ATP carrier (Anc) of Schizosaccharomyces pombe (Sp). The cDNA clone was isolated from a cDNA library of this fission yeast by complementation of a Saccharomyces cerevisiae (Sc) strain defective in adenine nucleotide carrier. The predicted amino acid (aa) sequence (322 aa) shared similarity with the known Anc sequences. It is more closely related to Neurospora crassa (Nc) Anc than to ScAnc1, 2, or 3 or Kluyveromyces lactis (Kl) Anc. Hybridization experiments with ordered libraries of Sp genomic DNA led to the physical mapping (chromosome II, NotI-B region) and the isolation of the Sp ANC1 gene. We also conclude that a single-copy gene encodes the Sp Anc.
我们描述了粟酒裂殖酵母（Sp）线粒体ADP/ATP载体（Anc）编码的cDNA和基因组克隆的分离与测序。通过对腺嘌呤核苷酸载体缺陷的酿酒酵母（Sc）菌株进行互补，从该裂殖酵母的cDNA文库中分离出cDNA克隆。预测的氨基酸（aa）序列（322个aa）与已知的Anc序列具有相似性。它与粗糙脉孢菌（Nc）Anc的关系比与ScAnc1、2或3或乳酸克鲁维酵母（Kl）Anc的关系更密切。用Sp基因组DNA的有序文库进行杂交实验，实现了物理图谱定位（第二条染色体，NotI-B区域）并分离出Sp ANC1基因。我们还得出结论，单个拷贝基因编码Sp Anc。
