Okuno S, Akazawa S, Yasuhi I, Kawasaki E, Matsumoto K, Yamasaki H, Matsuo H, Yamaguchi Y, Nagataki S
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Horm Metab Res. 1995 May;27(5):231-4. doi: 10.1055/s-2007-979946.
Insulin resistance involves impaired activities of the glucose transport system in insulin target tissues. We therefore investigated the GLUT4 glucose transporter protein in adipose tissues from the pregnant women with normal glucose tolerance and from women with gestational diabetes mellitus, and compared these to nonpregnant women. Three groups of women were studied: nonpregnant women with normal glucose tolerance (N = 6), pregnant women with normal glucose tolerance (N = 6, gestational week 38.0 +/- 0.3), and pregnant women with gestational diabetes mellitus (N = 3, gestational week 38.6 +/- 0.3). The abdominal subcutaneous adipose tissues obtained from each group were subjected to analysis of the GLUT4 glucose transporter protein. The presence of the GLUT4 glucose transporter protein in the three groups was quantitatively determined by Western blot analysis of detergent-soluble adipose tissue extracts using anti-GLUT4 antibody. GLUT4 glucose transporter protein concentration in the adipose tissue of pregnant women were significantly lower than that in nonpregnant women, and this difference was more profound in women with gestational diabetes mellitus. We demonstrated that the content of GLUT4 protein was decreased in adipose tissue from normal pregnancy compared to nonpregnant women.
胰岛素抵抗涉及胰岛素靶组织中葡萄糖转运系统的活性受损。因此，我们研究了糖耐量正常的孕妇和妊娠期糖尿病女性的脂肪组织中的葡萄糖转运蛋白4（GLUT4），并将其与未怀孕女性进行比较。研究了三组女性：糖耐量正常的未怀孕女性（N = 6）、糖耐量正常的孕妇（N = 6，妊娠周数38.0±0.3）和妊娠期糖尿病孕妇（N = 3，妊娠周数38.6±0.3）。对每组获取的腹部皮下脂肪组织进行GLUT4葡萄糖转运蛋白分析。使用抗GLUT4抗体对去垢剂可溶的脂肪组织提取物进行蛋白质免疫印迹分析，定量测定三组中GLUT4葡萄糖转运蛋白的存在情况。孕妇脂肪组织中的GLUT4葡萄糖转运蛋白浓度显著低于未怀孕女性，且这种差异在妊娠期糖尿病女性中更为明显。我们证明，与未怀孕女性相比，正常妊娠脂肪组织中GLUT4蛋白的含量降低。
