Pope L M, Reed K E, Payne S M
Department of Microbiology, University of Texas at Austin 78712, USA.
Infect Immun. 1995 Sep;63(9):3642-8. doi: 10.1128/iai.63.9.3642-3648.1995.
Growth of Shigella spp. in the presence of the bile salt deoxycholate or chenodeoxycholate enhanced the bacterial invasion of HeLa cells. Growth in the presence of other structurally similar bile salts or detergents had little or no effect. Deoxycholate-enhanced invasion was not observed when bacteria were exposed to deoxycholate at low temperatures or when chloramphenicol was added to the growth medium, indicating that bacterial growth and protein synthesis are required. Increased invasion is associated with the presence of an intact Shigella virulence plasmid and is correlated with increased secretion of a set of proteins, including the Ipa proteins, to the outer membrane and into the growth medium. The increased invasion induced by the bile salts appears to be due to increased adherence. The enhanced adherence was specific to Shigella spp., since the enteroinvasive Escherichia coli strains tested did not exhibit the effect in response to growth in bile salts.
在胆盐脱氧胆酸盐或鹅脱氧胆酸盐存在的情况下，志贺氏菌属的生长增强了其对HeLa细胞的侵袭。在其他结构相似的胆盐或去污剂存在的情况下生长几乎没有影响或没有影响。当细菌在低温下暴露于脱氧胆酸盐或在生长培养基中添加氯霉素时，未观察到脱氧胆酸盐增强的侵袭，这表明细菌生长和蛋白质合成是必需的。侵袭增加与完整的志贺氏菌毒力质粒的存在有关，并且与一组蛋白质（包括Ipa蛋白）向细胞膜外和生长培养基中的分泌增加相关。胆盐诱导的侵袭增加似乎是由于粘附增加。增强的粘附对志贺氏菌属具有特异性，因为测试的肠侵袭性大肠杆菌菌株在胆盐中生长时没有表现出这种效应。
