鼬獾幽门螺杆菌同基因脲酶阴性突变体的构建与鉴定
Construction and characterization of an isogenic urease-negative mutant of Helicobacter mustelae.
作者信息
Solnick J V, Josenhans C, Suerbaum S, Tompkins L S, Labigne A
机构信息
Division of Infectious Diseases, University of California, Davis, School of Medicine, USA.
出版信息
Infect Immun. 1995 Sep;63(9):3718-21. doi: 10.1128/iai.63.9.3718-3721.1995.
Abstract
Helicobacter mustelae infects the ferret stomach and provides an opportunity to study pathogenic determinants of a Helicobacter species in its natural host. We constructed an isogenic urease-negative mutant of H. mustelae which produced no detectable urease and showed a reduced acid tolerance. This mutant provides an opportunity to further evaluate the role of urease in the pathogenesis of Helicobacter infection.
摘要
幽门螺杆菌（Helicobacter mustelae）感染雪貂胃部，为研究幽门螺杆菌在其自然宿主中的致病决定因素提供了契机。我们构建了一种幽门螺杆菌的同基因脲酶阴性突变体，该突变体不产生可检测到的脲酶，且耐酸性降低。此突变体为进一步评估脲酶在幽门螺杆菌感染发病机制中的作用提供了机会。
相似文献
1
Construction and characterization of an isogenic urease-negative mutant of Helicobacter mustelae.鼬獾幽门螺杆菌同基因脲酶阴性突变体的构建与鉴定
Infect Immun. 1995 Sep;63(9):3718-21. doi: 10.1128/iai.63.9.3718-3721.1995.
2
Inability of an isogenic urease-negative mutant stain of Helicobacter mustelae to colonize the ferret stomach.鼬獾幽门螺杆菌同基因尿素酶阴性突变株无法在雪貂胃中定殖。
Infect Immun. 1995 Sep;63(9):3722-5. doi: 10.1128/iai.63.9.3722-3725.1995.
3
Development of a PCR-restriction fragment length polymorphism assay using the nucleotide sequence of the Helicobacter hepaticus urease structural genes ureAB.利用肝螺杆菌脲酶结构基因ureAB的核苷酸序列开发一种聚合酶链反应-限制性片段长度多态性检测方法。
J Clin Microbiol. 1998 Sep;36(9):2447-53. doi: 10.1128/JCM.36.9.2447-2453.1998.
4
Characterization of NikR-responsive promoters of urease and metal transport genes of Helicobacter mustelae.鉴定幽门螺旋杆菌尿素酶和金属转运基因的 NikR 反应启动子。
Biometals. 2010 Feb;23(1):145-59. doi: 10.1007/s10534-009-9275-7. Epub 2009 Nov 6.
5
Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster.比氏螺杆菌脲酶基因簇的克隆与特性分析
DNA Seq. 2002 Dec;13(6):321-31. doi: 10.1080/1042517021000039230.
6
Urease-mediated destruction of bacteria is specific for Helicobacter urease and results in total cellular disruption.脲酶介导的细菌破坏对幽门螺杆菌具有特异性，并导致细胞完全破裂。
FEMS Immunol Med Microbiol. 1994 Oct;9(4):273-80. doi: 10.1111/j.1574-695X.1994.tb00362.x.
7
Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter.对一种新鉴定出的未培养幽门螺杆菌脲酶基因的分子分析。
Infect Immun. 1994 May;62(5):1631-8. doi: 10.1128/iai.62.5.1631-1638.1994.
8
Cloning, expression and sequencing of Helicobacter felis urease genes.猫幽门螺杆菌脲酶基因的克隆、表达及测序
Mol Microbiol. 1993 Jul;9(2):323-33. doi: 10.1111/j.1365-2958.1993.tb01693.x.
9
Random mutagenesis to identify novel Helicobacter mustelae virulence factors.通过随机诱变鉴定新型雪貂幽门螺杆菌毒力因子。
FEMS Immunol Med Microbiol. 2007 Jul;50(2):257-63. doi: 10.1111/j.1574-695X.2006.00207.x. Epub 2007 May 22.
10
Protein Hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in Helicobacter pylori and Helicobacter mustelae.蛋白质Hpn：幽门螺杆菌和鼬獾螺杆菌中一种富含组氨酸的金属结合多肽的克隆与特性分析
Infect Immun. 1995 Jul;63(7):2682-8. doi: 10.1128/iai.63.7.2682-2688.1995.
引用本文的文献
1
Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen.幽门犬螺杆菌的比较基因组学和蛋白质组学研究，一种致溃疡和致癌的胃部病原体。
BMC Genomics. 2010 Mar 10;11:164. doi: 10.1186/1471-2164-11-164.
2
Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health.家畜和非人灵长类动物中的胃螺旋杆菌及其对人类健康的意义。
Clin Microbiol Rev. 2009 Apr;22(2):202-23, Table of Contents. doi: 10.1128/CMR.00041-08.
3
Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis.用于区分猫螺杆菌、比氏螺杆菌和所罗门螺杆菌的多重聚合酶链反应检测法。
J Clin Microbiol. 2004 Mar;42(3):1115-22. doi: 10.1128/JCM.42.3.1115-1122.2004.
4
Emergence of diverse Helicobacter species in the pathogenesis of gastric and enterohepatic diseases.多种幽门螺杆菌在胃和肝肠疾病发病机制中的出现。
Clin Microbiol Rev. 2001 Jan;14(1):59-97. doi: 10.1128/CMR.14.1.59-97.2001.
5
Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis.通过随机插入诱变鉴定幽门螺杆菌的毒力基因
Infect Immun. 1999 May;67(5):2433-40. doi: 10.1128/IAI.67.5.2433-2440.1999.
6
Development of a PCR-restriction fragment length polymorphism assay using the nucleotide sequence of the Helicobacter hepaticus urease structural genes ureAB.利用肝螺杆菌脲酶结构基因ureAB的核苷酸序列开发一种聚合酶链反应-限制性片段长度多态性检测方法。
J Clin Microbiol. 1998 Sep;36(9):2447-53. doi: 10.1128/JCM.36.9.2447-2453.1998.
7
Inability of an isogenic urease-negative mutant stain of Helicobacter mustelae to colonize the ferret stomach.鼬獾幽门螺杆菌同基因尿素酶阴性突变株无法在雪貂胃中定殖。
Infect Immun. 1995 Sep;63(9):3722-5. doi: 10.1128/iai.63.9.3722-3725.1995.
本文引用的文献
1
Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange.幽门螺杆菌和鼬源螺杆菌flaB鞭毛蛋白基因的克隆、遗传特征分析以及通过电穿孔介导的等位基因交换构建幽门螺杆菌flaA和flaB阴性突变体
J Bacteriol. 1993 Jun;175(11):3278-88. doi: 10.1128/jb.175.11.3278-3288.1993.
2
The phylogenetic position of Helicobacter nemestrinae.猪幽门螺杆菌的系统发育位置。
Int J Syst Bacteriol. 1993 Apr;43(2):386-7. doi: 10.1099/00207713-43-2-386.
3
Cloning, expression and sequencing of Helicobacter felis urease genes.猫幽门螺杆菌脲酶基因的克隆、表达及测序
Mol Microbiol. 1993 Jul;9(2):323-33. doi: 10.1111/j.1365-2958.1993.tb01693.x.
4
An uncultured gastric spiral organism is a newly identified Helicobacter in humans.一种未培养的胃螺旋状微生物是人类新发现的一种幽门螺杆菌。
J Infect Dis. 1993 Aug;168(2):379-85. doi: 10.1093/infdis/168.2.379.
5
Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter.对一种新鉴定出的未培养幽门螺杆菌脲酶基因的分子分析。
Infect Immun. 1994 May;62(5):1631-8. doi: 10.1128/iai.62.5.1631-1638.1994.
6
Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori.胃内pH值对幽门螺杆菌在悉生仔猪中脲酶依赖性定植的影响。
Infect Immun. 1994 Sep;62(9):3604-7. doi: 10.1128/iai.62.9.3604-3607.1994.
7
A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach.通过等位基因交换诱变构建的幽门螺杆菌脲酶阴性突变体缺乏定殖于裸鼠胃部的能力。
Infect Immun. 1994 Aug;62(8):3586-9. doi: 10.1128/iai.62.8.3586-3589.1994.
8
Helicobacter pylori requires an acidic environment to survive in the presence of urea.幽门螺杆菌在有尿素存在的情况下需要酸性环境来生存。
Infect Immun. 1995 May;63(5):1669-73. doi: 10.1128/iai.63.5.1669-1673.1995.
9
Automated micromeasurement of urea using urease and the Berthelot reaction.使用脲酶和伯索洛反应自动微量测定尿素。
Am J Clin Pathol. 1970 Dec;54(6):828-32. doi: 10.1093/ajcp/54.6.828.
10
Attempt to fulfil Koch's postulates for pyloric Campylobacter.尝试满足幽门弯曲菌的科赫法则。
Med J Aust. 1985 Apr 15;142(8):436-9. doi: 10.5694/j.1326-5377.1985.tb113443.x.
Zotero 插件