Andrutis K A, Fox J G, Schauer D B, Marini R P, Murphy J C, Yan L, Solnick J V
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139, USA.
Infect Immun. 1995 Sep;63(9):3722-5. doi: 10.1128/iai.63.9.3722-3725.1995.
Eight ferrets specific-pathogen-free for Helicobacter mustelae were given, per dose, approximately 3.0 x 10(7) CFU of either the wild-type parent strain of H. mustelae (NCTC 12032) (two ferrets) the isogenic urease-negative mutant strain of H. mustelae (10::Tn3Km) (four ferrets), or sterile culture broth (two ferrets). Infection status was monitored by endoscopic gastric biopsy for urease activity, histopathology, and culture and by serology at 3, 6, 10, and 21 weeks. All ferrets were necropsied at 25 weeks. Both negative control ferrets remained uninfected, both ferrets receiving the H. mustelae wild-type parent strain became infected after two doses of the organism, and all four ferrets given two doses of the isogenic urease-negative mutant strain of H. mustelae remained uninfected throughout the 6-month study. Histopathology correlated with infection status. H. mustelae-infected ferrets exhibited diffuse mononuclear inflammation in the subglandular region and the lamina propria of the gastric mucosa, while uninfected ferrets showed no or minimal inflammation. These results suggest that urease activity is essential for colonization of the ferret stomach by H. mustelae.
八只无特定病原体的雪貂，每只每次给予约3.0×10⁷CFU的幽门螺杆菌（Helicobacter mustelae），其中两只雪貂给予野生型亲本菌株（NCTC 12032），四只雪貂给予同基因脲酶阴性突变菌株（10::Tn3Km），另外两只雪貂给予无菌培养液。在第3、6、10和21周通过内镜胃活检监测脲酶活性、组织病理学和培养情况以及血清学，以监测感染状况。所有雪貂在25周时进行尸检。两只阴性对照雪貂未被感染，两只接受幽门螺杆菌野生型亲本菌株的雪貂在给予两剂该菌后被感染，而在整个6个月的研究中，所有四只给予两剂幽门螺杆菌同基因脲酶阴性突变菌株的雪貂均未被感染。组织病理学与感染状况相关。感染幽门螺杆菌的雪貂在胃黏膜的腺下区域和固有层表现出弥漫性单核细胞炎症，而未感染的雪貂则无炎症或仅有轻微炎症。这些结果表明，脲酶活性对于幽门螺杆菌在雪貂胃中的定植至关重要。
