Bagchi M, Van Wijnen A, Katar M, Merriman H, Lian J, Stein J, Stein G, Maisel H
Department of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
J Cell Biochem. 1995 May;58(1):1-5. doi: 10.1002/jcb.240580102.
This study examines matrix and nonmatrix nuclear proteins of the rabbit lens epithelial cells. The nuclear matrix proteins were isolated by modified Penman technique, which requires presence of detergents and nucleases, whereas nonmatrix nuclear proteins were obtained by high salt extraction. The data from these experiments revealed presence of DNA binding activities for SP-1 and OCT-1 proteins in both matrix and non-matrix compartments of rabbit lens epithelial cells. Comparison of the relative abundance of SP-1 and OCT-1 binding activities in nuclear matrix and nonmatrix fractions suggest the distribution between these two compartments is cell type specific and possibly related to the control of cell growth.
本研究检测兔晶状体上皮细胞的核基质蛋白和非核基质蛋白。核基质蛋白采用改良的彭曼技术分离，该技术需要使用去污剂和核酸酶，而非核基质蛋白则通过高盐提取获得。这些实验数据显示，兔晶状体上皮细胞核基质和非核基质部分中均存在SP-1和OCT-1蛋白的DNA结合活性。核基质和非核基质部分中SP-1和OCT-1结合活性相对丰度的比较表明，这两个部分之间的分布具有细胞类型特异性，可能与细胞生长的调控有关。
