Bagchi M, Ansari S A, Lindenmuth D M, van Wijnen A J, Lian J, Stein J L, Stein G S
Department of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, MI 48201, USA.
Mol Biol Rep. 1998 Jan;25(1):13-9. doi: 10.1023/a:1006886110771.
Association of transcription factors with the nuclear matrix represents a mechanism by which nuclear architecture may influence transcriptional control of gene expression. This investigation examines nuclear matrix associated proteins (NMP's) isolated from ocular lens epithelial cells by monitoring DNA binding activities using consensus oligonucleotides recognized by the transcription factors YY1, AML-1, AP-1, SP-1 and ATF. The nuclear matrix fractions tested included an immortilized human lens epithelial cell line containing the SV40 large T-antigen, and two mouse lens epithelial cell lines derived from either a normal mouse or a cataract mouse. A rabbit epidermal epithelial cell line and HeLa cells were also included in this study for comparison. The data from these experiments reveal that ubiquitously represented and tissue restricted regulatory proteins are associated with nuclear matrix of lens epithelial cells. The functional significance of the nuclear matrix association of these transcription factors remains to be determined. However, our findings raise the possibility that the transcription factors associated with the nuclear matrix could have specific roles in gene regulation and eye tissue development.
转录因子与核基质的结合代表了一种机制，通过该机制核结构可能影响基因表达的转录调控。本研究通过使用转录因子YY1、AML-1、AP-1、SP-1和ATF识别的共有寡核苷酸监测DNA结合活性，来检测从眼晶状体上皮细胞中分离出的核基质相关蛋白（NMP）。所测试的核基质组分包括含有SV40大T抗原的永生化人晶状体上皮细胞系，以及源自正常小鼠或白内障小鼠的两种小鼠晶状体上皮细胞系。本研究还纳入了兔表皮上皮细胞系和HeLa细胞作为对照。这些实验的数据表明，广泛存在的和组织特异性的调节蛋白与晶状体上皮细胞的核基质相关。这些转录因子与核基质结合的功能意义尚待确定。然而，我们的研究结果提出了一种可能性，即与核基质相关的转录因子可能在基因调控和眼组织发育中具有特定作用。
