Ni Z, Smogorzewski M, Massry S G
Department of Medicine, University of Southern California, School of Medicine, Los Angeles, USA.
Kidney Int. 1995 Jun;47(6):1624-9. doi: 10.1038/ki.1995.226.
Chronic renal failure (CRF) is associated with increased calcium content of, and impaired lipase release from lipid cells. This has been attributed to a rise in the cytosolic calcium ([Ca2+]i) of these cells. However, data on [Ca2+]i of lipid cells in CRF and on the mechanisms responsible for such an abnormality are lacking. To study this issue we examined the [Ca2+]i and ATP content of lipid cells and Vmax of Na(+)-K(+)-ATPase and Ca2+ ATPase of membrane preparation and Na(+)-Ca2+ exchange of membrane vesicles of adipocytes from normal rats, 6 week CRF, CRF normocalcemic parathyroidectomized (CRF-PTX) and CRF, and normal rats treated with verpamil (CRF-V, normal-V). [Ca2+]i in adipocytes of CRF rats was higher (199 +/- 8.5 nM) and ATP lower (2.9 +/- 0.31 nmol/10(6) cells) than in normal (120 +/- 4.3 nM; 5.7 +/- 0.27 nmol/10(6) cells), CRF-PTX (128 +/- 4.7 nM; 5.8 +/- 0.39 nmol/10(6) cells), normal-V (121 +/- 3.2 nM; 5.3 +/- 0.36 nmol/10(6) cells), CRF-V (123 +/- 7.4 nM; 5.5 +/- 0.30 mmol/10(6) cells). Vmax Ca2+ ATPase and the activity of Na(+)-K(+)-ATPase and of Na(+)-Ca2+ exchanger were reduced in CRF rats as compared to the other four groups of rats. The values in normal, CRF-PTX, CRF-V and normal-V rats were not different.(ABSTRACT TRUNCATED AT 250 WORDS)
慢性肾衰竭（CRF）与脂质细胞中钙含量增加以及脂质细胞脂肪酶释放受损有关。这归因于这些细胞胞质钙（[Ca2+]i）的升高。然而，关于CRF中脂质细胞的[Ca2+]i以及导致这种异常的机制的数据尚缺乏。为研究此问题，我们检测了正常大鼠、6周CRF大鼠、CRF正常血钙甲状旁腺切除（CRF-PTX）大鼠以及CRF大鼠和用维拉帕米处理的正常大鼠（CRF-V、正常-V）脂肪细胞的[Ca2+]i、ATP含量、膜制剂的Na(+)-K(+)-ATP酶和Ca2+ATP酶的Vmax以及膜囊泡的Na(+)-Ca2+交换。与正常（120±4.3 nM；5.7±0.27 nmol/10(6)个细胞）、CRF-PTX（128±4.7 nM；5.8±0.39 nmol/10(6)个细胞）、正常-V（121±3.2 nM；5.3±0.36 nmol/10(6)个细胞）、CRF-V（123±7.4 nM；5.5±0.30 mmol/10(6)个细胞）相比，CRF大鼠脂肪细胞中的[Ca2+]i更高（199±8.5 nM）而ATP更低（2.9±0.31 nmol/10(6)个细胞）。与其他四组大鼠相比，CRF大鼠中Ca2+ATP酶的Vmax以及Na(+)-K(+)-ATP酶和Na(+)-Ca2+交换体的活性降低。正常、CRF-PTX、CRF-V和正常-V大鼠的值无差异。（摘要截断于250字）
