Klin M, Smogorzewski M, Massry S G
Division of Nephrology, University of Southern California School of Medicine, Los Angeles 90033, USA.
Am J Physiol. 1995 Jul;269(1 Pt 1):G103-9. doi: 10.1152/ajpgi.1995.269.1.G103.
Chronic renal failure (CRF) is associated with increased Ca2+ content of liver and reduced hepatic lipase activity. This has been attributed to a rise in cytosolic Ca2+ ([Ca2+]i) of the hepatocytes, but data on this issue are lacking. We examined the [Ca2+]i and ATP content of hepatocytes as well as the activity of Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase), Ca(2+)-ATPase, and Na+/Ca2+ exchanger of hepatic membrane vesicles from normal rats, animals with 6 wk of CRF, CRF normocalcemic parathyroidectomized (CRF-PTX) rats, and CRF and normal animals treated with verapamil (CRF-V, normal-V). [Ca2+]i in hepatocytes of CRF rats was higher (281 +/- 7.4 nM) and ATP lower (6.4 +/- 1.8 nmol/mg protein) than in normal (209 +/- 5.3 nM; 12.5 +/- 0.89 nmol/mg protein), CRF-PTX (212 +/- 1.0 nM; 13.7 +/- 0.79 nmol/mg protein), normal-V (215 +/- 2.3 nM; 14.2 +/- 0.77 nmol/mg protein), and CRF-V rats (209 +/- 7.4 nM; 14.8 +/- 0.72 nmol/10(6) cells). The Na(+)-K(+)-ATPase, the maximal velocity of Ca(2+)-ATPase, and the activity of the Na+/Ca2+ exchanger were reduced, whereas the Michaelis constant of Ca(2+)-ATPase was increase in CRF rats compared with the other four groups of rats. The values in the latter groups were not different.(ABSTRACT TRUNCATED AT 250 WORDS)
慢性肾衰竭（CRF）与肝脏中钙离子（Ca2+）含量增加及肝脂肪酶活性降低有关。这被归因于肝细胞胞质钙离子浓度（[Ca2+]i）升高，但关于此问题的数据尚缺乏。我们检测了正常大鼠、患CRF 6周的动物、CRF正常血钙甲状旁腺切除（CRF-PTX）大鼠以及用维拉帕米治疗的CRF和正常动物（CRF-V、正常-V）的肝细胞[Ca2+]i、ATP含量以及肝细胞膜囊泡的钠钾腺苷三磷酸酶（Na(+)-K(+)-ATPase）、钙ATP酶（Ca(2+)-ATPase）和钠/钙交换体的活性。与正常（209±5.3 nM；12.5±0.89 nmol/mg蛋白）、CRF-PTX（212±1.0 nM；13.7±0.79 nmol/mg蛋白）、正常-V（215±2.3 nM；14.2±0.77 nmol/mg蛋白）和CRF-V大鼠（209±7.4 nM；14.8±0.72 nmol/10(6)个细胞）相比，CRF大鼠肝细胞中的[Ca2+]i更高（281±7.4 nM），ATP更低（6.4±1.8 nmol/mg蛋白）。与其他四组大鼠相比，CRF大鼠的Na(+)-K(+)-ATPase、Ca(2+)-ATPase的最大速度以及钠/钙交换体的活性降低，而Ca(2+)-ATPase的米氏常数增加。后几组的值没有差异。（摘要截短于250词）
