Aaberge I S, Eng J, Lermark G, Løvik M
Department of Vaccine, National Institute of Public Health, Oslo, Norway.
Microb Pathog. 1995 Feb;18(2):141-52. doi: 10.1016/s0882-4010(95)90125-6.
Animal models of pneumococcal infection are important to evaluate the protective capacity of new vaccine candidates. We have established a method to prepare and store the experimental inoculum without loss of virulence or number of bacteria. This allows a standardized inoculum from the same culture batch to be used in several experiments. Pneumococci were cultured to mid-logarithmic growth phase in Todd-Hewitt broth with 17% fetal calf serum. The bacterial broth was distributed into smaller volumes and immediately frozen on liquid nitrogen and stored at -70 degrees C. We have tested the virulence of five different pneumococcal serotypes in BALB/c, C57BL/6, and NIHS mice using inocula prepared by this method and stored without loss of virulence for up to 4 years. Serotypes 1, 4, 5 and 8 were highly virulent for the strains of mice tested whereas type 6B showed lower virulence and a peculiar, protracted course of infection. There were no clear differences in virulence between the different strains of mice with the exception of serotype 6B, which showed higher virulence in BALB/c and NIHS mice than in C57BL/6 mice.
肺炎球菌感染的动物模型对于评估新型候选疫苗的保护能力很重要。我们已经建立了一种制备和储存实验接种物的方法，该方法不会导致毒力丧失或细菌数量减少。这使得来自同一培养批次的标准化接种物可用于多个实验。将肺炎球菌在含有17%胎牛血清的托德-休伊特肉汤中培养至对数生长期中期。将细菌肉汤分装成较小体积，立即在液氮中冷冻，并储存在-70℃。我们使用通过这种方法制备并在毒力无损失的情况下储存长达4年的接种物，测试了五种不同肺炎球菌血清型在BALB/c、C57BL/6和NIHS小鼠中的毒力。血清型1、4、5和8对所测试的小鼠品系具有高毒力，而6B型显示出较低的毒力和独特的、病程迁延的感染过程。除了6B血清型在BALB/c和NIHS小鼠中比在C57BL/6小鼠中显示出更高的毒力外，不同小鼠品系之间在毒力上没有明显差异。
