Keast J R
Department of Physiology and Pharmacology, University of Queensland, Australia.
Neuroscience. 1995 Jun;66(3):655-62. doi: 10.1016/0306-4522(94)00595-v.
Pelvic ganglia contain a mixture of sympathetic and parasympathetic neurons which are innervated by either lumbar (sympathetic) or sacral (parasympathetic) preganglionic axons, respectively. However, until recently no stain for these axon terminals has been available. In the present study of the male rat major pelvic ganglion, a ganglion which supplies axons to the lower urinary and digestive tracts and internal reproductive organs, the total population of preganglionic axon terminals was immunostained using an antiserum against synaptophysin, a protein associated with all small synaptic vesicles (such as the presumptive cholinergic vesicles present in all preganglionic terminals). Selective bilateral lesions of either the hypogastric or pelvic nerves, which carry the sympathetic and parasympathetic preganglionic axons, respectively, were carried out and three to seven days later ganglia were examined immunohistochemically for the distribution of residual synaptophysin-positive terminals. Neurons remaining innervated following hypogastric nerve lesion were therefore classified as parasympathetic and those innervated after pelvic nerve section were defined as sympathetic. These two cell groups are present in approximately equal proportions. Double-staining immunofluorescence to identify which transmitters or peptides are present in either sympathetic or parasympathetic neurons showed that the majority (approximately 75%) of sympathetic neurons are presumed to be noradrenergic (i.e. contain tyrosine hydroxylase immunoreactivity) and are also immunoreactive for neuropeptide Y; the remainder contain vasoactive intestinal peptide-immunoreactivity but not tyrosine hydroxylase and may be cholinergic. Parasympathetic neurons were virtually all non-noradrenergic (tyrosine hydroxylase negative) and were also of two histochemical types, with some neurons containing neuropeptide Y- and others containing vasoactive intestinal peptide-immunoreactivity.(ABSTRACT TRUNCATED AT 250 WORDS)
盆腔神经节包含交感和副交感神经元的混合体，它们分别由腰段（交感）或骶段（副交感）节前轴突支配。然而，直到最近还没有针对这些轴突终末的染色方法。在本项对雄性大鼠主要盆腔神经节的研究中，该神经节为下尿路、消化道和内生殖器官提供轴突，使用抗突触素的抗血清对节前轴突终末的总数进行免疫染色，突触素是一种与所有小突触囊泡相关的蛋白质（如所有节前终末中存在的假定胆碱能囊泡）。分别对携带交感和副交感节前轴突的腹下神经或盆腔神经进行选择性双侧损伤，三到七天后对神经节进行免疫组织化学检查，以观察残留的突触素阳性终末的分布。因此，腹下神经损伤后仍有神经支配的神经元被归类为副交感神经元，盆腔神经切断后有神经支配的神经元被定义为交感神经元。这两类细胞群的比例大致相等。通过双重免疫荧光染色来确定交感或副交感神经元中存在哪些递质或肽，结果显示，大多数（约75%）交感神经元被认为是去甲肾上腺素能的（即含有酪氨酸羟化酶免疫反应性），并且对神经肽Y也有免疫反应性；其余的含有血管活性肠肽免疫反应性，但不含酪氨酸羟化酶，可能是胆碱能的。副交感神经元几乎都是非去甲肾上腺素能的（酪氨酸羟化酶阴性），并且也有两种组织化学类型，一些神经元含有神经肽Y，另一些含有血管活性肠肽免疫反应性。（摘要截短于250字）
