Ghailani N, Guillemin C, Vigneron C
Service de Chimie Hématologique, Centre Régional de Transfusion Sangunie de Nancy, Vandeuver-lés-Nancy, France.
Nouv Rev Fr Hematol (1978). 1995;37(2):141-7.
The purpose of this study was to determine the protein composition of the vesicles released from senescent erythrocytes and its variation during their experimental conservation at +4 degrees C in citrate phosphate dextrose (CPD), over a period of 8 weeks. Techniques employed included electron microscopy, SDS-PAGE and immunoblotting. Electron microscopic observation of senescent erythrocytes showed the simultaneous release of one or several vesicles of varying size and shape, while close examination of individual vesicles revealed a slack membrane structure and the presence of band 3 protein. SDS-PAGE and immunoblotting showed the vesicles to be composed mainly of band 3 and its breakdown products and to be lacking in spectrin. Use of specific antibodies demonstrated the presence of free haemoglobin, immunoglobulin G (IgG) and fragment C3b of complement. During conservation for 8 weeks, the concentration of band 3 protein decreased, while the concentrations of IgG and C3b increased and there was no apparent variation in haemoglobin levels.
本研究的目的是确定衰老红细胞释放的囊泡的蛋白质组成，以及在8周的时间内，它们于4℃在枸橼酸盐-磷酸盐-葡萄糖（CPD）中进行实验保存期间其蛋白质组成的变化。所采用的技术包括电子显微镜、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法。衰老红细胞的电子显微镜观察显示，同时释放出一个或几个大小和形状各异的囊泡，而对单个囊泡的仔细检查发现其膜结构松弛且存在带3蛋白。SDS-PAGE和免疫印迹法显示，这些囊泡主要由带3及其降解产物组成，且缺乏血影蛋白。使用特异性抗体证实存在游离血红蛋白、免疫球蛋白G（IgG）和补体片段C3b。在保存8周期间，带3蛋白的浓度降低，而IgG和C3b的浓度升高，血红蛋白水平无明显变化。
