Corrier D E, Nisbet D J, Scanlan C M, Hollister A G, Deloach J R
USDA, Agriculture Research Service, College Station, Texas 77845, USA.
Poult Sci. 1995 Jun;74(6):916-24. doi: 10.3382/ps.0740916.
A continuous-flow culture system was used to isolate and maintain a mixed culture of cecal bacteria from adult broilers composed of 29 bacterial strains representing 10 genera. Broiler chicks were treated with the mixed culture in the drinking water on the day of hatch and challenged orally with 10(4) Salmonella typhimurium 2 d after treatment. The experiment was repeated in four separate trials using newly hatched chicks. The concentration of propionic acid and total volatile fatty acid (VFA) in the cecal contents was determined 2 d after treatment and at 10 d of age. Compared with controls, the number of treated chicks that were cecal culture-positive for Salmonella decreased (P < .01) in each of the trials. Additionally, the number of Salmonella in the cecal contents of the treated chicks at 10 d of age was decreased (P < .005) compared with controls in each trial. The decreased number of Salmonella in the cecal contents of the 10-d-old treated chicks was correlated with elevated concentrations of propionic acid (P < .05) and total VFA (P < .1) in the cecal contents of the treated chicks 2 d after treatment. The results indicated that VFA-producing bacteria present in the mixed culture became rapidly established in the ceca of the treated chicks and effectively increased resistance to S. typhimurium challenge.
采用连续流培养系统从成年肉鸡盲肠中分离并维持一种混合培养物，该混合培养物由代表10个属的29种细菌菌株组成。在孵化当天，给雏鸡饮用含有混合培养物的水，并在处理后2天口服10(4)鼠伤寒沙门氏菌进行攻毒。使用新孵化的雏鸡在四个独立试验中重复该实验。在处理后2天和10日龄时测定盲肠内容物中丙酸和总挥发性脂肪酸（VFA）的浓度。与对照组相比，在每个试验中，经处理的雏鸡盲肠培养物中沙门氏菌呈阳性的数量均减少（P < 0.01）。此外，在每个试验中，与对照组相比，10日龄经处理雏鸡盲肠内容物中的沙门氏菌数量均减少（P < 0.005）。10日龄经处理雏鸡盲肠内容物中沙门氏菌数量的减少与处理后2天经处理雏鸡盲肠内容物中丙酸浓度升高（P < 0.05）和总VFA浓度升高（P < 0.1）相关。结果表明，混合培养物中存在的产VFA细菌在经处理雏鸡的盲肠中迅速定殖，并有效提高了对鼠伤寒沙门氏菌攻毒的抵抗力。
