Piquer F J, Sell J L, Jeffrey M J, Reynolds D L, Akinc S, Kaiser M
Department of Animal Science, Iowa State University, Ames 50011, USA.
Poult Sci. 1995 Jun;74(6):998-1010. doi: 10.3382/ps.0740998.
Two 21-d experiments were conducted to document the effects of an early immunologic stress and changes in dietary ME(n) on selected characteristics of immune function of newly hatched turkeys. Eight treatments were included in each experiment. Treatments were the result of complete factorial arrangements of two types of injection and four isonitrogenous diets. Turkeys in both experiments were injected i.p. with .5, .5, and .2 mL of saline (SAL) or .5, .5, and .2 mL of a solution of Escherichia coli lipopolysaccharide (LPS) (100 micrograms LPS/mL SAL) at 1, 3, and 5 d of age, respectively. In Experiment 1, two diets were formulated to contain 2,800 kcal ME(n)/kg. One was a corn-soybean meal based diet (CSBM) and the other contained 8% Solkafloc (SKF). A third diet (3,100 kcal ME(n)/kg) was formulated by substituting 8% sucrose (SUC) for the 8% SKF. The fourth diet (HE) included in Experiment 1 was formulated to contain 3,700 kcal ME(n)/kg. The CSBM and SUC diets and two additional diets were tested in Experiment 2. The latter were the CSBM diet containing 74.5 mg ibuprofen/kg (IBU) and a corn-soybean meal diet formulated to contain 3,100 kcal ME(n)/kg (CS31). Concentrations of plasma IgG and jejunal IgG and IgA were not affected by injection or diet. Age-related changes in Ig concentrations were consistently observed in Experiments 1 and 2. Injection with LPS reduced the number or responses of blood leukocytes to mitogens at 8 d of age (P < .01), as compared with samples from turkeys injected with SAL. Leukocytes in whole blood samples from turkeys fed the HE diet responded less to LPS stimulation than those fed the SUC diet (P < .01). Injection with LPS did not markedly affect the characteristics of immune function studied, and feeding a diet with 3,100 kcal ME(n)/kg and 28.5% crude protein did not measurably affect the characteristics of immune function of young turkeys.
进行了两项为期21天的试验，以记录早期免疫应激以及日粮代谢能（n）变化对新孵化火鸡免疫功能特定指标的影响。每项试验包含8种处理。处理方式是两种注射类型和四种等氮日粮的完全析因安排。两项试验中的火鸡分别在1日龄、3日龄和5日龄时腹腔注射0.5、0.5和0.2 mL生理盐水（SAL）或0.5、0.5和0.2 mL大肠杆菌脂多糖（LPS）溶液（100微克LPS/mL SAL）。在试验1中，配制了两种日粮，代谢能（n）含量均为2800千卡/千克。一种是以玉米-豆粕为基础的日粮（CSBM），另一种含有8%的索尔卡弗洛克（SKF）。第三种日粮（3100千卡ME(n)/千克）是用8%的蔗糖（SUC）替代8%的SKF配制而成。试验1中的第四种日粮（HE）的代谢能（n）含量为3700千卡/千克。CSBM日粮和SUC日粮以及另外两种日粮在试验2中进行了测试。后两种日粮分别是含有74.5毫克布洛芬/千克的CSBM日粮（IBU）和配制的代谢能（n）含量为3100千卡/千克的玉米-豆粕日粮（CS31）。血浆IgG、空肠IgG和IgA的浓度不受注射或日粮的影响。在试验1和试验2中均持续观察到Ig浓度随年龄的变化。与注射SAL的火鸡样本相比，注射LPS使8日龄时血液白细胞对丝裂原的数量或反应降低（P<0.01）。饲喂HE日粮的火鸡全血样本中的白细胞对LPS刺激的反应低于饲喂SUC日粮的火鸡（P<0.01）。注射LPS对所研究的免疫功能指标没有显著影响，饲喂代谢能（n）含量为3100千卡/千克且粗蛋白含量为28.5%的日粮对小火鸡的免疫功能指标也没有明显影响。
