Erkine A M, Szent-Gyorgyi C, Simmons S F, Gross D S
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130, USA.
Yeast. 1995 May;11(6):573-80. doi: 10.1002/yea.320110607.
We present the upstream sequences of HSP82 and HSC82, two closely related, but differentially regulated, heat-shock genes of Saccharomyces cerevisiae. Several dozen potential regulatory elements are identified within each upstream region; interestingly, only a few are conserved between the two genes. These include a consensus heat-shock element, an upstream repressor element, and a consensus TATA element. A search for motifs known actively to position nucleosomes in vitro revealed that such sequences are three- to seven-fold enriched within each promoter; a comparable enrichment is seen near the 3' end of each transcription unit. Located approximately 1100 bp upstream of HSC82 is an open reading frame (ORF) of 255 amino acids; approximately 800 bp upstream of HSP82 is an ORF of 132 amino acids. The latter ORF contains several conserved ankyrin motifs and appears to be expressed under normal growth conditions. Finally, we show by clamped homogeneous electric field gel electrophoresis that the two genetic loci map to different chromosomes: HSP82 to chromosome XVI and HSC82 to chromosome XIII. The sequences have been deposited in the GenBank database under Accession Numbers U20323 and U20349.
我们展示了酿酒酵母中两个密切相关但调控方式不同的热休克基因HSP82和HSC82的上游序列。在每个上游区域内鉴定出了几十种潜在的调控元件；有趣的是，这两个基因之间只有少数元件是保守的。这些元件包括一个共有热休克元件、一个上游阻遏元件和一个共有TATA元件。对已知在体外能积极定位核小体的基序进行搜索发现，此类序列在每个启动子内富集了三到七倍；在每个转录单元的3'端附近也观察到了类似的富集。在HSC82上游约1100 bp处是一个由255个氨基酸组成的开放阅读框（ORF）；在HSP82上游约800 bp处是一个由132个氨基酸组成的ORF。后一个ORF包含几个保守的锚蛋白基序，并且似乎在正常生长条件下表达。最后，我们通过钳位均匀电场凝胶电泳表明，这两个基因座定位于不同的染色体上：HSP82定位于第十六号染色体，HSC82定位于第十三号染色体。这些序列已以登录号U20323和U20349存入GenBank数据库。
