Yoshida M, Shimma Y, Uno I, Toh-e A
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Yeast. 1994 Mar;10(3):371-6. doi: 10.1002/yea.320100309.
We have cloned NES24 using a temperature-sensitive nes24-1 mutant as a host and sequenced a 3162 bp XhoI-EcoRI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24-1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature-sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.
我们利用温度敏感型nes24 - 1突变体作为宿主克隆了NES24，并对包含NES24基因的3162 bp XhoI - EcoRI DNA片段进行了测序。计算机分析表明，该片段包含一个1806 bp的开放阅读框，这是互补nes24 - 1突变所必需的。我们在NES24基因上游区域发现了SUP8，从而将NES24基因定位在第十三号染色体上。蛋白质同源性搜索表明NES24编码一种新蛋白质。NES24基因的破坏导致温度敏感型生长。该序列已以登录号D15052存入DDBJ/EmBL/GenBank数据库。
