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[用喹吖因氮芥染色后,在两栖类有尾目动物波氏肋突螈(Pleurodeles poireti Gervais)有丝分裂染色体上显示Q带。与沃氏肋突螈(Pleurodeles waltii)的比较]

[Demonstration of Q bands on mitotic chromosomes of the amphibian urodele Pleurodeles poireti Gervais, after staining with quinacrine mustard. Comparison with Pleurodeles waltii].

作者信息

Bailly S

出版信息

Ann Genet. 1977 Dec;20(4):227-31.

PMID:76459
Abstract

Preparations of metaphase chromosomes of the amphibian urodele Pleurodeles poireti were obtained by squashing cells from tailbuds of ten days old embryos which had been in 50% acetic acid. The Q-bands karyotype is described. Frequently the induced secondary constrictions exhibit a bright fluorescence, as do the centromeres, except for that of chromosome XII. In contrast, satellites on chromosomes III, IV, and XI exhibit little fluorescence. This pattern is compared with that in Pleurodeles waltlii. Differences are observable in centromeres of chromosomes III and XII and the proximal part of the long arm of chromosome VII, differences which can be used as chromosome marker in case of hybridization.

摘要

通过挤压取自十天大胚胎尾芽且已置于50%醋酸中的细胞,获得了两栖类有尾目动物波氏肋突螈的中期染色体标本。描述了Q带核型。诱导产生的次缢痕经常呈现出明亮的荧光,着丝粒也是如此,但第十二号染色体的着丝粒除外。相比之下,第三、四和十一号染色体上的随体荧光较弱。将这种模式与沃氏肋突螈的模式进行了比较。在第三和十二号染色体的着丝粒以及七号染色体长臂的近端部分可观察到差异,这些差异在杂交情况下可用作染色体标记。

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