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[经喹吖因芥末染色后,在两栖动物美西螈有丝分裂染色体上观察到的Q带的定位及意义(作者译)]

[Localization and significance of the Q bands observed on mitotic chromosomes of the amphibian Pleurodeles waltlii Michah. after coloration by quinacrine mustare (author's transl)].

作者信息

Bailly S

出版信息

Chromosoma. 1976 Jan 27;54(1):61-8. doi: 10.1007/BF00331833.

Abstract

Metaphase plates from tailbuds of Pleurodeles waltlii embryos (stage 34) with or without preceding cold treatment were obtained by squashing followed by quinacrine mustard staining. In both cases, caryograms were established and sites of Q bands located.--Most of the secondary constrictions exhibit very high fluorescence. In general, it is the same for most centromeric parts, but their fluorescence is quenched very strongly by cold treatment.--The proximal part of the long arm of chromosome VII and satellites of chromosomes III and XI exhibit dull fluorescence. All these sites are compared with heterochromatin localization. The relation between banding and base composition or non-histone proteins interactions is discussed.

摘要

通过挤压并随后进行喹吖因芥子染色,获得了有或没有经过前期冷处理的疣螈胚胎(34期)尾芽的中期板。在这两种情况下,都建立了核型图并确定了Q带的位置。——大多数次缢痕显示出非常高的荧光。一般来说,大多数着丝粒部分也是如此,但它们的荧光在冷处理后被强烈淬灭。——染色体VII长臂的近端部分以及染色体III和XI的随体显示出暗淡的荧光。所有这些位点都与异染色质定位进行了比较。文中还讨论了带型与碱基组成或非组蛋白蛋白质相互作用之间的关系。

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