Poljak M, Orlowska J, Cerar A
Institute of Microbiology, Medical Faculty of Ljubljana, Slovenia.
Anticancer Res. 1995 May-Jun;15(3):965-9.
The etiology and pathogenesis of esophageal squamous cell papillomas (ESCP), rare benign tumors of human esophagus, are still controversial. Chronic mucosal irritation and infection with human papillomaviruses (HPV) are two proposed etiologies. To investigate these hypotheses, we screened 29 ESCPs from 28 patients originating from Slovenia and Poland for HPV infection using in situ hybridization (ISH) and polymerase chain reaction (PCR). No evidence of HPV DNA was found using ISH. By PCR, the presence of HPV DNA was detected in only one lesion using two different HPV L1 consensus primer sets. The restriction fragment analyses of PCR product showed patterns unique to HPV type 6. All other ESCPs were successfully amplified only with internal control human beta-globin primers. Our results show that HPV DNA is not frequently detectable in ESCPs, even when highly sensitive methods like PCR are used and that other pathogenetic mechanisms are more important in the etiology of ESCPs.
食管鳞状细胞乳头状瘤（ESCP）是人类食管罕见的良性肿瘤，其病因和发病机制仍存在争议。慢性黏膜刺激和人乳头瘤病毒（HPV）感染是两种提出的病因。为了研究这些假说，我们使用原位杂交（ISH）和聚合酶链反应（PCR）对来自斯洛文尼亚和波兰的28例患者的29个ESCP进行了HPV感染筛查。使用ISH未发现HPV DNA的证据。通过PCR，仅在一个病变中使用两种不同的HPV L1共有引物组检测到HPV DNA的存在。PCR产物的限制性片段分析显示出HPV 6型独特的模式。所有其他ESCP仅用内部对照人β-珠蛋白引物成功扩增。我们的结果表明，即使使用PCR等高灵敏度方法，在ESCP中也不常检测到HPV DNA，并且其他致病机制在ESCP的病因中更为重要。
