Chang F, Syrjänen S, Shen Q, Cintorino M, Santopietro R, Tosi P, Syrjänen K
Department of Clinical Pathology, Kuopio University Hospital, POB 1777, FIN-70211 Kuopio, Finland.
Anticancer Res. 2000 Sep-Oct;20(5C):3935-40.
Certain viruses, notably human papillomavirus (HPV), cytomegalovirus (CMV), herpes simplex virus (HSV) and Epstein-Barr virus (EBV), are known to produce tumors in animals and cell transformation in vitro and they have been implicated in the pathogenesis of human cancers. All these viruses are also known to infect the esophagus. This study was aimed to determine whether these viruses play any causal role in the etiology of esophageal squamous cell carcinoma.
A series of 103 esophageal squamous cell carcinomas derived from patients in the high-incidence area of northern China were analyzed by DNA in situ hybridization and polymerase chain reaction (PCR) for the presence of HPV DNA sequences and, using immunohistochemistry, for the demonstration of CMV, HSV and EBV infections.
Six (5.8%) of the 103 tumors were found to contain HPV 16, 18 or 30 DNA sequences. HPV types 6, 11 and 53 were not detected in any of the cases. Amplified HPV DNA sequences were found in 17 out of 101 (16.8%) carcinoma specimens by PCR with L1 consensus primers. None of the 103 carcinomas tested was immunohistochemically positive for CMV, HSV or EBV.
Our results confirmed the HPV involvement in esophageal carcinomas and provided further evidence to support a causal association of HPV infection with esophageal squamous cell carcinoma. However, the three herpesviruses, CMV, HSV and EBV, are highly unlikely to be involved in the pathogenesis of this malignancy in the high-incidence area of China.
已知某些病毒，尤其是人乳头瘤病毒（HPV）、巨细胞病毒（CMV）、单纯疱疹病毒（HSV）和爱泼斯坦-巴尔病毒（EBV）可在动物体内诱发肿瘤并在体外引起细胞转化，且它们与人类癌症的发病机制有关。所有这些病毒也已知会感染食管。本研究旨在确定这些病毒在食管鳞状细胞癌的病因学中是否起任何因果作用。
采用DNA原位杂交和聚合酶链反应（PCR）分析了来自中国北方高发地区患者的103例食管鳞状细胞癌，以检测HPV DNA序列的存在，并采用免疫组织化学方法检测CMV、HSV和EBV感染情况。
103例肿瘤中有6例（5.8%）被发现含有HPV 16、18或30 DNA序列。所有病例均未检测到HPV 6、11和53型。用L1通用引物通过PCR在101例癌标本中的17例（16.8%）中发现了扩增的HPV DNA序列。103例检测的癌组织中，CMV、HSV或EBV免疫组织化学检测均为阴性。
我们的结果证实了HPV与食管癌有关，并提供了进一步的证据支持HPV感染与食管鳞状细胞癌之间的因果关系。然而，在中国高发地区，CMV、HSV和EBV这三种疱疹病毒极不可能参与这种恶性肿瘤的发病机制。
