Toraya T, Maoka T, Tsuji H, Kobayashi M
Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
Appl Environ Microbiol. 1995 May;61(5):1799-804. doi: 10.1128/aem.61.5.1799-1804.1995.
Inhibitors of bacterial origins of starfish oocyte maturation were sought to obtain biologically active substances which act on either hormonal signal transduction or cell cycle regulation. An oocyte maturation-inhibiting substance found in culture fluid of a Bacillus species was purified to homogeneity. This substance possessed the nature of a detergent and specifically inhibited 1-methyladenine-induced oocyte maturation (50% inhibitory concentration, 3.3 microM) but not dithiothreitol-induced maturation. Its total structure was established to be the lactone of 3-hydroxy-13-methyltetradecanoyl-Glu-Leu-Leu-Val-Asp-Leu -Leu through COOH of the carboxy-terminal Leu. This structure is identical to surfactin, although although the configuration of the substance's amino acid residues has not yet been determined. Surfactin was shown to be identical with this substance in its inhibitory effect on starfish oocyte maturation as well as its chromatographic and electrophoretic properties. Therefore, it was concluded that the oocyte maturation-inhibiting substance produced by a Bacillus species is surfactin.
为了获得作用于激素信号转导或细胞周期调控的生物活性物质，人们寻找了海星卵母细胞成熟细菌起源的抑制剂。从一种芽孢杆菌属的培养液中发现的一种抑制卵母细胞成熟的物质被纯化至同质。该物质具有去污剂的性质，能特异性抑制1-甲基腺嘌呤诱导的卵母细胞成熟（50%抑制浓度，3.3微摩尔），但不抑制二硫苏糖醇诱导的成熟。其完整结构经确定为通过羧基末端亮氨酸的羧基形成的3-羟基-13-甲基十四烷酰基-Glu-Leu-Leu-Val-Asp-Leu-Leu的内酯。尽管该物质氨基酸残基的构型尚未确定，但此结构与表面活性素相同。表面活性素在对海星卵母细胞成熟的抑制作用以及其色谱和电泳性质方面与该物质相同。因此，可以得出结论，一种芽孢杆菌属产生的抑制卵母细胞成熟的物质是表面活性素。
